| 乙型肝炎病毒X基因的克隆与鉴定 |
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| 引用本文： | 苏辉,李玉军,佟松,翟爱霞,李小光,张凤民. 乙型肝炎病毒X基因的克隆与鉴定[J]. 哈尔滨医科大学学报, 2007, 41(3): 221-223 |
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| 作者姓名： | 苏辉 李玉军 佟松 翟爱霞 李小光 张凤民 |
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| 作者单位： | 哈尔滨医科大学,微生物学教研室,黑龙江,哈尔滨,150081;哈尔滨医科大学,微生物学教研室,黑龙江,哈尔滨,150081;哈尔滨医科大学,微生物学教研室,黑龙江,哈尔滨,150081;哈尔滨医科大学,微生物学教研室,黑龙江,哈尔滨,150081;哈尔滨医科大学,微生物学教研室,黑龙江,哈尔滨,150081;哈尔滨医科大学,微生物学教研室,黑龙江,哈尔滨,150081 |
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| 摘 要： | 目的 构建乙型肝炎病毒X基因原核表达质粒pQE30-HBx,以进一步研究其基因产物的功能及致病机制.方法 以肝炎患者血清DNA为模板,用PCR方法扩增乙型肝炎病毒X基因,构建乙型肝炎病毒X基因原核表达质粒pQE30-HBx,经酶切电泳验证重组质粒的正确性,并对X基因进行测序鉴定.结果 PCR结果显示,扩增片断大小约465 bp,与预期相同.重组质粒经酶切电泳判断有目的片段插入,方向正确,经测序证实插入片段是乙型肝炎病毒X基因,编码框无误.结论 成功构建乙型肝炎病毒X基因重组质粒pQE30-HBx.
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| 关 键 词： | 乙型肝炎病毒 X基因 原核表达系统 |
| 文章编号： | 1000-1905（2007）03-0221-03 |
| 修稿时间： | 2007-04-13 |
Clone and identification of hepatitis B Virus X gene |
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| SU Hui, LI Yu-jun, TONG Song, et al. Clone and identification of hepatitis B Virus X gene[J]. Journal of Harbin Medical University, 2007, 41(3): 221-223 |
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| Authors: | SU Hui LI Yu-jun TONG Song et al |
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| Affiliation: | Department of Microbiology, Harbin Medical University, Harbin 150081, China |
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| Abstract: | Objective To construct the prokaryotic expression plasmid for the X gene of hepatitis B virus(HBx).Methods The X gene of hepatitis B virus was amplified from serum of hepatitis B patients by PCR method,and inserted into prokaryotic expression vector pQE30;The structure of recombinant pQE30-HBx plasmid was confirmed by restrict endonuclease digestion and sequencing analysis.Results The 465bp DNA fragment of X gene was obtained and inserted exactly in recombinant pQE30-HBx plasmia.Sequencing confirmed that the cloned gene was HBx.Conclusion The recombinant pQE30-HBx plasmia had been constructed successfully. |
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| Keywords: | hepatitis B virus X gene prokaryotic expression |
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