FAK和MMP-9蛋白在胃癌组织中的表达及相关性研究

目的 检测基质金属蛋白酶9(MMP-9)和黏着斑激酶(FAK)在胃癌中的表达,并探讨其相关性.方法 采用免疫组织化学方法,观察60例胃癌及肿瘤与正常组织交界处FAK和MMP-9的表达情况.结果 60例胃癌组织中FAK阳性及强阳性表达率为73.3%(44/60)、56.7%(34/60),MMP-9的阳性表达率为85%(51/60);60例相应交界组织中,FAK阳性及强阳性表达率为56.7%(34/60)、26.7%(16/60),MMP-9阳性表达率为56.7%(34/60).MMP-9和FAK阳性表达率和阳性强度均以胃癌中为高.在癌组织中,MMP-9和FAK在低分化组中的表达高于高、中分化组,在淋巴结转移组中较无淋巴转移组表达高,浸润程度越深表达越高.MMP-9阳性表达强度与FAK的阳性表达强度之间呈显著正相关(P＜0.05).结论 胃癌中FAK与MMP-9的表达密切相关,可作为胃癌生物学行为预测的辅助指标.

Study on the correlation between expressions of focal adhesion kinase and metalloproteinase in gastric cancerous tissues

Objective To detect the expressions of matrix metalloproteinase9(MMP-9) and focal adhesion kinase(FAK) in human gastric cancer,and to study their correlation.Methods Immunohistochemical technique was used to detect the expressions of MMP-9 and FAK in 60 gastric cancers and adjacent gastric tissues.Results Positive expression rate of MMP-9 in gastric cancer was 85%(51/60).Positive expression and strong positive expression rate of FAK were 73.3%(44/60),56.7%(34/60),respectively.In the adjacent gastric tissue to cancerous tissue,positive expression rate of MMP-9 was 36.7%(22/60),positive expression rate and strong positive expression rate of FAK were 56.7%(34/60),26.7%(16/60),respectively.Positive expression rates and levels of MMP-9 and FAK in gastric cancers were significantly higher than those in adjacent gastric tissues.In cancerous tissue,the expressions of MMP-9 and FAK were significantly higher in cases of poor differeniated tissues and with lymphnodes metastasis.The deeper the cancer infiltrated,the higher the positive expression rates showed.There was a significant correlation between MMP-9 and FAK expressions(P<0.01).Conclusion There was connection between the expressions of MMP-9 and FAK.Detection of FAK and MMP-9 expressions can be useful for evaluating the biological behavior of gastric cancer.