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| TACO与细胞膜具有非依赖肌动蛋白方式的结合 | |
| 引用本文： | 黄鹏,金萌萌,曲国蕃,唐佩福,王岩松,郝克强,王玉学.TACO与细胞膜具有非依赖肌动蛋白方式的结合[J].哈尔滨医科大学学报,2007,41(3):241-243,256. |
| 作者姓名： | 黄鹏 金萌萌 曲国蕃 唐佩福 王岩松 郝克强 王玉学 |
| 作者单位： | 1. 中国人民解放军总医院,骨科,北京,100853 2. 哈尔滨医科大学第二临床医学院,骨科 3. 哈尔滨医科大学第三临床医学院,骨科,黑龙江,哈尔滨,150081 |
| 摘 要： | 目的 探讨TACO与细胞膜之间的相互作用.方法 利用免疫荧光分析检测内源性TACO在细胞内的分布情况,然后利用细胞膜内膜表面展示分析和差速离心分离亚细胞组分分析检测TACO蛋白与细胞膜的结合,以及该结合与细胞膜上肌动蛋白纤维之间的相互关系.结果 TACO主要分布在细胞膜和核膜附近区域.结论 TACO与细胞膜具有非依赖肌动蛋白方式的结合,这一结果为阐释TACO在细胞吞噬初始阶段的功能提供了有力的实验依据. |
| 关 键 词： | TACO 细胞膜 肌动蛋白 |
| 文章编号： | 1000-1905（2007）03-0241-03 |
| 修稿时间： | 2007-01-11 |
Binding between TACO and cell membrane is in a non-actin related way |
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| HUANG Peng, JIN Meng-meng, QU Guo-fan, et al.Binding between TACO and cell membrane is in a non-actin related way[J].Journal of Harbin Medical University,2007,41(3):241-243,256. | |
| Authors: | HUANG Peng JIN Meng-meng QU Guo-fan |
| Institution: | Department of Orthopaedics, PEA General Hospital, Beijing 100853, China |
| Abstract: | Objective To analyse the interrelationship of tryptophan-aspartate-containing coat protein(TACO) and cellular membrane,and try to understand the molecular mechnism and function.Methods Immunofluorescence analysis was used to test the endogenous distribution of TACO in the cell.And then the inner membrane surface display analysis and differential centrifugation separation inferior celelular component was used to detect the binding between TACO and cellular membrane and their binding's relationship with the actin-binding proteins fibers.Results TACO was mainly distributed near the cellular membrane and nuclear membrane.Conclusion The binding between TACO and cell membrane is non-actin related.The result can provide evidence for exploring the function of TACO at the early stage of cell phagocytose. |
| Keywords: | TACO plasma membrane actin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |