| 小鼠肝微粒体CYP450酶系检测方法的实验研究 |
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| 引用本文： | 郑良朴,林薇,叶蕻芝. 小鼠肝微粒体CYP450酶系检测方法的实验研究[J]. 福建中医学院学报, 2006, 16(4): 50-52 |
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| 作者姓名： | 郑良朴 林薇 叶蕻芝 |
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| 作者单位： | 福建中医学院中西医结合研究院,福建,福州,350108 |
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| 摘 要： | 对小鼠肝微粒体CYP450酶系检测方法进行研究，应用差速离心法获取小鼠肝微粒体，采用Bradford法检测肝微粒体蛋白浓度，用分光光度法检测CYP450酶系含量及活性，实验表明该方法具有便捷可靠，重复性好等特点。
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| 关 键 词： | 分光光度法 |
| 文章编号： | 1004-5627（2006）04-0050-03 |
| 收稿时间： | 2006-06-12 |
| 修稿时间： | 2006-06-12 |
Experimental Study on Method of Measuring Cytochrome P450 in Mouse Liver Microsome |
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| ZHENG Liang-pu,LIN Wei,YE Hong-zhi. Experimental Study on Method of Measuring Cytochrome P450 in Mouse Liver Microsome[J]. Journal of Fujian College of Traditional Chinese Medicine, 2006, 16(4): 50-52 |
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| Authors: | ZHENG Liang-pu LIN Wei YE Hong-zhi |
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| Affiliation: | Fujian Academy of Integrative Medicine, FCTCM, Fuzhou, Fujian 350108, China |
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| Abstract: | To study the method of measuring cytochrome P450(CYP450) in mouse liver microsome.Mouse liver microsome was obtained by different velocity centrifugation,the concentration of protein of liver microsome was measured by Bradford, and the activity of mice CYP450 was detected by spectrophotometer.The result showed that this method is easy and reliable,and has good reproducibility. |
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| Keywords: | Bradford CYP450 |
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