| 模拟缺血预适应对原代培养乳鼠窦房结细胞内钙及L-型钙电流的影响 |
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| 引用本文： | 张倩,宋治远,仝识非,黄骥. 模拟缺血预适应对原代培养乳鼠窦房结细胞内钙及L-型钙电流的影响[J]. 第三军医大学学报, 2005, 27(6): 494-496 |
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| 作者姓名： | 张倩 宋治远 仝识非 黄骥 |
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| 作者单位： | 第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所,重庆,400038;第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所,重庆,400038;第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所,重庆,400038;第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所,重庆,400038 |
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| 基金项目： | 国家自然科学基金资助项目(30070314)~~ |
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| 摘 要： | 目的 观察模拟缺血预适应(IP)对培养乳鼠窦房结细胞内Ca2 及I -LCa的影响,探讨IP对窦房结细胞的保 护机制。方法 取培养2d的乳鼠窦房结细胞,随机分为:①对照组;②模拟缺血 再灌注(I R)组;③模拟IP组。以免疫荧 光技术标记细胞内Ca2 ,通过激光共聚焦显微镜检测荧光强度变化;以全细胞膜片钳技术检测I -LCa。结果 模拟IP能显 著降低I R后窦房结细胞内Ca2 荧光强度,增加相应电压下的I- LCa峰值,使电流 电压曲线相对下移。结论 模拟IP能 减轻I R引起的窦房结细胞内Ca2 超载及I -LCa抑制。
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| 关 键 词： | 窦房结细胞 缺血预适应 钙离子 L-型钙电流 |
| 文章编号： | 1000-5404(2005)06-0494-03 |
| 修稿时间： | 2004-11-03 |
Effects of mimic ischemic preconditioning on [Ca2+]i and L-type calcium current of cultured sinoatrial node cells of neonatal rat |
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| ZHANG Qian,SONG Zhi-yuan,TONG Shi-fei,HUANG Ji. Effects of mimic ischemic preconditioning on [Ca2+]i and L-type calcium current of cultured sinoatrial node cells of neonatal rat[J]. Acta Academiae Medicinae Militaris Tertiae, 2005, 27(6): 494-496 |
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| Authors: | ZHANG Qian SONG Zhi-yuan TONG Shi-fei HUANG Ji |
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| Abstract: | Objective To study the effects of mimic ischemic preconditioning (IP) on i and I-LCa of sinoatrial node cells and explore the protective effects of IP. Methods Cells were randomized to three groups: control, ischemia/reperfusion (I/R), IP. After labeled with fura4, fluorescence intensity of i was studied with laser confocal microscopy and I-LCa was recorded by whole-cell patch clamp technology. Results IP significantly reduced the fluorescence intensity of i and enhanced the peak of I-LCa as compared with I/R, shifted the I-V curve to more negative value. Conclusion IP can reduce the overload of i caused by I/R and increase I-LCa weakened by I/R. |
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| Keywords: | sinoatrial node cell ischemic preconditioning calcium ion L-type calcium current |
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