2型糖尿病患者血清Vaspin水平的变化及其临床意义

目的 检测正常糖调节和新诊断的2型糖尿病的血清Vaspin水平,并探讨Vaspin与体重指数(BMI),年龄,性别,糖、脂代谢及胰岛素敏感性的相关性.方法 采用ELISA检测66例2型糖尿病患者(DM组)及48名正常人(对照组)的空腹血清Vaspin,测定受试者的BMI、腰臀比(WHR)、脂肪百分比;同时检测血糖、糖化血红蛋白(HbA1C)、血脂和胰岛素水平.结果 (1)DM组血清Vaspin:非肥胖者为(0.65±0.13)μg/L,肥胖者为(1.13±0.25)μg/L;对照组血清Vaspin:非肥胖者为(0.38±0.18)μg/L,肥胖者为(0.95±0.11)μg/L.2组肥胖者血清Vaspin水平均高于同组非肥胖者(P均＜0.01).(2)DM组男性血清Vaspin水平为(0.76±0.22)μg/L,女性为(0.92±0.35)μg/L.对照组男性血清Vaspin水平为(0.48±0.14)μg/L,女性为(1.05±0.21)μg/L.2组血清Vaspin水平女性均高于男性(P均＜0.01).(3)直线相关分析发现,血清Vaspin浓度与BMI、WHR、脂肪百分比、空腹胰岛素(FINS)、胰岛素敏感指数(ISI)相关(r分别为0.365、0.214、0.238、0.183,0.147,P均＜0.05).用多元逐步回归分析发现性别(R2=0.161,P＜0.01)、ISI(R2=0.183,P＜0.01)、WHR(R2=0.216,P＜0.01)为血清Vaspin的独立相关因素.结论 肥胖者的血清Vaspin水平显著升高,且与WHR、性别、ISI呈独立相关.推测Vaspin可能参与构成胰岛素抵抗综合征的病理生理基础.

Abstract：

Objective To detect serum Vaspin level in subjects with normal glucose regulation(NGR)and newly-diagnosed type 2 diabetes mellitus (T2DM) ,and to investigate the association between serum Vaspin and body mass index (BMI), age, gender, glycometabolism and lipemetabolism and insulin sensitivity index. Methods The fasting serum levels of Vaspin were measured in 48 normal controls and 66 T2DM patients using enzyme linked immunosorbent assay ( ELISA ). BMI, waist-hip ratio (WHR) and % body fat were measured,as well as fasting blood glucose (FBG), HBA1C, lipids and insulin levels. Results The fast serum Vaspin concentrations in the obese T2DM patients was ( 1.13 ±0. 25 ) μg/L,which were significantly higher than that in the non-obese T2DM patients (0. 65 ± 0. 13 ) μg/L (P ＜ 0. 01 ). In the NGR group, the fast serum Vaspin concentrations in the obese was (0. 95 ± 0. 11 ) μg/L, which were significantly higher than that in the non-obese (0. 38 ± 0. 18) μg/L( P ＜ 0. 01 ). In the T2DM group, serum Vaspin concentration in females ( [0. 92 ± 0. 35]μg/L) was higher than in males ([0.76 ± 0. 22] μg/L) (P ＜ 0.01 ). In the NGR group, serum Vaspin concentration in females was also higher than that in males ( [1.05 ± 0. 21] μg/L vs [0. 48 ± 0. 14] μg/L) )(P＜0. 01 ). Serum Vaspin level was positively correlated with BMI,WHR,% Body Fat,fasting insulin(FINS)and insulin sensitivity index (ISI) (r = 0. 365,0. 214,0. 238,0. 183 and 0. 147, respectively, Ps ＜ 0. 05 ). The multiple stepwise regression analysis showed that gender( R2 = 0. 161, P ＜ 0. 01 ), ISI ( R2 = 0. 183, P ＜ 0. 01 )and WHR( R2 = 0. 216, P ＜ 0. 01 ) were independent variables associated with Vaspin. Conclusion Serum vaspin level significantly increases in subjects with obesity, and independently associated with gender, ISI,WHR. These findings suggest that serum Vaspin may be involved in the pathophysiology of insulin resistance syndrome.

Serum Vaspin level in type 2 diabete patients and its clinical significance

Objective To detect serum Vaspin level in subjects with normal glucose regulation(NGR)and newly-diagnosed type 2 diabetes mellitus (T2DM) ,and to investigate the association between serum Vaspin and body mass index (BMI), age, gender, glycometabolism and lipemetabolism and insulin sensitivity index. Methods The fasting serum levels of Vaspin were measured in 48 normal controls and 66 T2DM patients using enzyme linked immunosorbent assay ( ELISA ). BMI, waist-hip ratio (WHR) and % body fat were measured,as well as fasting blood glucose (FBG), HBA1C, lipids and insulin levels. Results The fast serum Vaspin concentrations in the obese T2DM patients was ( 1.13 ±0. 25 ) μg/L,which were significantly higher than that in the non-obese T2DM patients (0. 65 ± 0. 13 ) μg/L (P ＜ 0. 01 ). In the NGR group, the fast serum Vaspin concentrations in the obese was (0. 95 ± 0. 11 ) μg/L, which were significantly higher than that in the non-obese (0. 38 ± 0. 18) μg/L( P ＜ 0. 01 ). In the T2DM group, serum Vaspin concentration in females ( [0. 92 ± 0. 35]μg/L) was higher than in males ([0.76 ± 0. 22] μg/L) (P ＜ 0.01 ). In the NGR group, serum Vaspin concentration in females was also higher than that in males ( [1.05 ± 0. 21] μg/L vs [0. 48 ± 0. 14] μg/L) )(P＜0. 01 ). Serum Vaspin level was positively correlated with BMI,WHR,% Body Fat,fasting insulin(FINS)and insulin sensitivity index (ISI) (r = 0. 365,0. 214,0. 238,0. 183 and 0. 147, respectively, Ps ＜ 0. 05 ). The multiple stepwise regression analysis showed that gender( R2 = 0. 161, P ＜ 0. 01 ), ISI ( R2 = 0. 183, P ＜ 0. 01 )and WHR( R2 = 0. 216, P ＜ 0. 01 ) were independent variables associated with Vaspin. Conclusion Serum vaspin level significantly increases in subjects with obesity, and independently associated with gender, ISI,WHR. These findings suggest that serum Vaspin may be involved in the pathophysiology of insulin resistance syndrome.