趋化因子CCL15在肝细胞肝癌组织中的表达及其临床意义

目的：研究趋化因子CCL15在肝细胞肝癌（HCC）组织中的表达及其临床意义，并对CCL15与肝癌的恶性行为之间关系进行研究。方法：80例HCC、80例癌旁组织和50例正常肝组织标本常规制作石蜡包埋切片，CCL15染色为SP免疫组化法。结果：HCC组织中的CCL15表达阳性率及其评分明显高于癌旁组织和正常肝组织 （P<0.05），在癌旁组织和正常肝组织之间没有差异；CCL15的蛋白表达与患者的性别、年龄、肝硬化、病理学分级、术前AFP浓度无明显关系（P>0.05），而与肿瘤的大小、癌栓、有无包膜以及TNM分期等有显著关系。结论：CCL15的表达与HCC的一些恶性临床病理特征密切相关，提示CCL15可能是反映HCC发生发展、侵袭、转移的一个重要的生物学指标。

Expression of CCL15 in Hepatocellular Carcinoma and Its Clinical Significance

Expression of CCL15 in Hepatocellular Carcinoma and Its Clinical SignificanceYueguo LI1, Ning ZHANG2Correspondence to: Ning ZHANG, E-mail: kesaqi@163.com1Department of Laboratory, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China2Central Laboratory, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, ChinaAbstract Objective: To study the expression of CCL15 in hepatocellular carcinoma (HCC) and its clinicopathologic signifi-cance, and to study the relationship between expression levels of CCL15 and malignant behaviors of HCC. Methods: Immunohisto-chemistry (SP method) was used to detect expression of CCL15 in paraffin-embedded sections from 80 cases of HCC, 80 samples of ad-jacent tissue and 50 samples of normal liver tissue. Results: The positive rates of CCL15 were significantly higher in HCC than in adja-cent tissue specimens and normal liver tissue ( P < 0.05 ). No significant difference was found between adjacent tissue specimens andnormal liver tissue ( P < 0.05). The expression of CCL15 was significantly correlated with tumor size and tumor thrombi in the portalvein ( P < 0.05 ), but not with sex, age, liver cirrhosis or the level of AFP ( P > 0.05 ). Conclusion: The expression of CCL15 was signif-icantly correlated with malignant behaviors of HCC, thus CCL15 might be an important biological marker in the oncogenesis, progres-sion, biological behavior and prognosis of HCC.Keywords Hepatocellular neoplasm; CCL15; Immunohistochemistry