miR-150在套细胞淋巴瘤中的表达及意义

  目的   探讨套细胞淋巴瘤（mantle cell lymphoma，MCL）中miR-150的表达情况及其临床意义。   方法   通过定量RT-PCR检测29例初治MCL患者及7例正常人外周血B细胞中miR-150和c-Myc的表达水平，探索miR-150和c-Myc表达之间的关系；利用RNAi阻断MCL细胞系Mino和HBL-2中c-Myc表达后，检测miR-150的变化，确定c-Myc是否参与miR-150的表达调控；抑制P493-6细胞表达c-Myc后，观察miR-150的变化，进一步明确miR-150是否受c-Myc调节；将pre-miR-150电转HBL-2细胞系，通过集落形成试验明确miR-150对细胞增殖的影响，Western blot检测c-Myb蛋白的变化。   结果   与正常人外周血B细胞相比，MCL患者低表达miR-150、过表达c-Myc，两者的表达呈负相关；阻断c-Myc后，Mino和HBL-2细胞的miR-150表达增加；抑制c-Myc表达后，P493-6细胞的miR-150表达增高；过表达miR-150后，HBL-2的c-Myb蛋白表达水平和集落形成能力下降。   结论   MCL患者低表达miR-150的原因可能与其c-Myc过表达有关。miR-150能够抑制MCL的增殖，在MCL的治疗中具有潜在价值。 

MiR-150 downregulation and its significance in mantle cell lymphoma

  Objective   This study explores the miR-150 expression and its clinical significance in mantle cell lymphoma (MCL).   Methods   The miR-150 and c-Myc expression was measured in 29 primary MCL tissue samples and 7 normal donors through quantitative real-time polymerase chain reaction. MiR-150 expression was detected in Mino and HBL-2 cells after c-Myc was knocked down by small interfering RNAs. MiR-150 was analyzed in tet-treated P493-6 cells with Myc turned off. The number of tumor colonies in the BL-2 cells transfected with pre-miR-150 was determined through colony formation assay, and c-Myb was detected through Western blot.   Results   Compared with the normal donors, miR-150 expression was significantly downregulated and c-Myc was considerably overexpressed in MCL. Moreover, MCLs with high Myc expression had a significantly low miR-29 expression. MiR-150 was upregulated in the Mino and HBL-2 cells with c-Myc knockdown. MiR-150 was evidently upregulated in P493-6 cells after c-Myc was turned off. MiR-150 overexpression suppressed colony formation and c-Myb expression of HBL-2.   Conclusion   MiR-150 is downregulated in MCL, which may be related to c-Myc overexpression. The recovery of miR-150 suppresses MCL cell survival. These results indicate that miR-150 may be a potential therapeutic target of MCL.