| 大肠埃希菌连续分离株对消毒剂-磺胺耐药基因的研究 |
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| 引用本文： | 王继东,周丽珍,钱小毛.大肠埃希菌连续分离株对消毒剂-磺胺耐药基因的研究[J].中国消毒学杂志,2007,24(4):331-333. |
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| 作者姓名： | 王继东 周丽珍 钱小毛 |
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| 作者单位： | 1. 无锡市第五人民医院,无锡,214073
2. 绍兴第二医院 |
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| 摘 要： | 目的了解大肠埃希菌连续分离株对消毒剂-磺胺耐药基因存在情况和耐药性。方法应用聚合酶链反应法对大肠埃希菌耐药株对消毒剂和磺胺耐药基因进行检测与分析。结果经对连续分离的68株大肠埃希菌中,检测出消毒剂-磺胺耐药基因(qacE△1与sulⅠ)阳性41株,检出率为60.3%。结论临床连续分离大肠埃希菌耐药株消毒剂-磺胺耐药基因检出率较高,提示临床抗药菌株可能同时对消毒剂存在抗药性。
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| 关 键 词： | 大肠埃希菌 消毒剂 磺胺 耐药基因 聚合酶链反应 |
| 文章编号： | 1001-7658（2007）04-0331-03 |
| 修稿时间： | 2006-12-06 |
STUDY ON DISINFECTANT-SULFANILAMIDE RESISTANCE GENES OF SUCCESSIVELY ISOLATED STRAINS OF Escherichia coli |
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| WANG Ji-dong,ZHOU Li-zhen,QIAN Xiao-mao.STUDY ON DISINFECTANT-SULFANILAMIDE RESISTANCE GENES OF SUCCESSIVELY ISOLATED STRAINS OF Escherichia coli[J].Chinese Journal of Disinfection,2007,24(4):331-333. |
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| Authors: | WANG Ji-dong ZHOU Li-zhen QIAN Xiao-mao |
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| Abstract: | Objective To know the presence of disinfectant-sulfanilamide resistance genes of successively isolated strains of Escherichia coli and their drug-resistance. Methods Polymerase chain reaction method was used to detect and analyze the disinfectant-sulfanilamide resistance genes of the drug-resistant strain of Escherichia coli. Results Of the 68 successively isolated strains of E.coli, 41 were positive to disinfectant-sulfanilamide resistance genes (qacEΔ1 and sulⅠ), with a detection rate of 60.3%. Conclusion The detection rate of disinfectant-sulfanilamide resistance genes in clinically successively isolated drug-resistant strains of E. coli was relatively high, indicating that clinical drug-resistant strains may have resistance to disinfectants simultaneously. |
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| Keywords: | Escherichia coli disinfectant sulfanilamide drug- resistance gene polymerase chain reaction |
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