侧脑室注射隐丹参酮单体对吗啡诱导的小鼠条件性位置偏爱效应形成和一氧化氮含量的影响

目的:探讨隐丹参酮单体对吗啡诱导的小鼠条件性位置偏爱(CPP)效应形成的影响及成瘾过程中一氧化氮(NO)递质水平改变与小鼠精神依赖的相关联系。方法:利用CPP实验方法,建立吗啡精神依赖模型。侧脑室注射(icv)隐丹参酮单体,观察其对吗啡诱导的CPP效应形成的影响。实验结束后用NO试剂盒测定小鼠全脑NO含量。结果:生理盐水组与吗啡组比较有显著的统计学意义(P<0.001);与吗啡组比较,隐丹参酮低、中、高剂量组(20ug.kg-1,40ug.kg-1,80ug.kg-1,icv)均能降低吗啡诱导的CPP得分,其中,中剂量组有明显的统计学意义(P<0.01),低剂量组和高剂量组有统计学意义(P<0.05),3个给药剂量组与生理盐水组比较均无统计学意义;生理盐水组与吗啡组小鼠全脑NO含量比较有明显的统计学意义(P<0.01)。隐丹参酮低、中、高剂量组均能提高小鼠全脑NO含量,其中,中剂量组和高剂量组与吗啡组比较有明显的统计学意义(P<0.01),与生理盐水组比较无统计学意义,低剂量组与生理盐水组和吗啡组比较均有统计学意义(P<0.05)。结论:隐丹参酮能抑制吗啡诱导的小鼠CPP效应形成,可能与抑制CPP效应形成过程中小鼠全脑NO递质含量的降低有关。

EFFECT OF INTRACEREBROVENTRICULAR ADMINISTRATION OF CRYPTOTANSHINONE ON THE ACQUISITION OF MORPHINE-INDUCED CONDITIONED PLACE PREFERENCE IN MORPHINE-SENSITIZED MICE AND NITRIC OXIDE CHANGE DURING THE ACQUISITION OF CPP

Objective:To study the effect of cryptotanshinone on the acquisition of morphine-induced conditioned place preference(CPP)and investigate the possible participation of nitric oxide(NO)in the acquisition of CPP in morphine-sensitized mice.Methods:Mice were randomly divided into 5 groups:NS group,Mor group,cryptotanshinone low,median and high dose groups(20 ug·kg-1,40 ug·kg-1,80 ug·kg-1,icv).Morphine-induced CPP model were established,with pre-administration of cryptotanshinone before sc morphine in the cryptotanshinone low,median and high dose groups,and NS group was sc saline.After the last testing(d9),the NO level of mice were measured with NO kit.Results:There was significant difference in CPP scores between NS group and Mor group(P<0.001).Compared with Mor group,the CPP scores of all three cryptotanshinone groups were significantly reduced(median dose group P<0.01,both low dose group and high dose group P<0.05),but no statistical differences were found compared with NS group.There was significant difference in the NO level between NS group and Mor group(P<0.01).The NO level of all three cryptotanshinone groups raised(compared to that of MOR group,both median dose group and high dose group P<0.01),no statistical differences were observed among median dose group,high dose group and NS group,but statistical difference was observed between NS group and low dose group,P<0.05.Conclusion:cryptotanshinone can inhibit the acquisition of morphine-induced CPP,which implies that NO is involved in the acquisition of morphine-induced CPP in morphine-sensitized mice.