JNK信号转导通路在NGF抗6-OHDA诱导PC12细胞凋亡中的作用

目的： 以6-羟基多巴胺(6-OHDA)作用于大鼠肾上腺嗜铬细胞瘤细胞(PC12 cells)以诱导其凋亡，然后在其中分别加入神经生长因子（NGF）及c-Jun氨基端激酶（JNK）阻断剂SP600125，研究在加入NGF后JNK的活性与凋亡的关系。方法: 实验分为对照组、6-OHDA组、NGF组、6-OHDA+NGF组、6-OHDA+JNK阻断剂SP600125组，以流式细胞分析法检测各组PC12细胞的凋亡率，以免疫印迹（Western blotting）法检测各组PC12细胞JNK的活化情况。结果: 6-OHDA导致PC12细胞凋亡，JNK1活性提高；预孵SP600125或NGF15min后再加入6-OHDA则PC12细胞凋亡率及JNK1活性均降低。结论: JNK1参与了6-OHDA致PC12细胞凋亡作用，NGF抗6-OHDA所诱导的PC12细胞凋亡作用与其抑制JNK的活化有关。

JNK signal transduction pathway in anti-apoptotic effect of NGF on PC12 cells treated with 6-hydroxydopamine

AIM: To investigate the anti-apoptotic effect of nerve growth factor (NGF) on PC12 cells and to observe the mechanism of signal transduction of JNK pathway. METHODS: PC12 cells were treated with 6-hydroxydopamine (6-OHDA) to induce cell apoptosis. NGF and SP600125, the c-Jun N-terminal kinase inhibitors, were added respectively in order to study the relationship between the activity of c-Jun N-terminal kinase and apoptosis of PC12 cells. The cells were divided into control group, 6- OHDA group, NGF group, 6-OHDA plus NGF group, NGF plus SP600125 group. The apoptotic rates of PC12 cells with different treatments were detected by flow cytometry and activities of JNK in PC12 cells were determined by Western blotting. RESULTS: 6-OHDA increased the apoptotic rate and activity of JNK1 in PC12 cells. Incubation with SP600125 or NGF for 15 min before adding 6-OHDA decreased the apoptotic rate of PC12 cells and activity of JNK1 in PC12 cells.CONCLUSION: Activity of JNK is involved in the pro-apoptotic effect of 6-OHDA on PC12 cells. Anti-apoptotic effect of NGF on PC12 cells affected by 6-OHDA is related to the decrease in the activity of JNK1.