| 重组腺相关病毒作为目标基因转移视网膜的载体的可行性研究 |
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| 引用本文： | 王建明,范雅稚,惠娜,熊蕾,冯海晓,孙乃学.重组腺相关病毒作为目标基因转移视网膜的载体的可行性研究[J].国际眼科杂志,2008,8(9):1740-1742. |
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| 作者姓名： | 王建明 范雅稚 惠娜 熊蕾 冯海晓 孙乃学 |
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| 作者单位： | 西安交通大学医学院第二附属医院眼科,中国陕西省西安市,710004 |
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| 摘 要： | 目的:探讨重组腺相关病毒基因(recombinant adeno-associ-ated virus gene,rAAV)载体转导绿荧光蛋白基因(green fluorescent protein,gfp)基因至视网膜的可行性。方法:18只家兔随机选取一眼玻璃体内注射rAAV-gfp,对侧眼作为对照。分别于注射后3,7,14d摘除眼球进行视网膜铺片观察视网膜的荧光。结果:家兔玻璃体注射rAAV-gfp后视网膜细胞浆内可见荧光点,提示gfp基因被有效转导至视网膜并进行荧光表达。结论:rAAV是一个可靠、简便易行的目标基因转移视网膜的载体。
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| 关 键 词： | 重组腺相关病毒 绿荧光蛋白基因 基因转染 视网膜 |
A feasibility study of recombinant adeno-associated virus(rAAV) as a vector for transferring a target gene to retina |
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| Jian-Ming Wang,Ya-Zhi Fan,Na Hui,Lei Xiong,Hai-Xiao Feng,Nai-Xue Sun.A feasibility study of recombinant adeno-associated virus(rAAV) as a vector for transferring a target gene to retina[J].International Journal of Ophthalmology,2008,8(9):1740-1742. |
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| Authors: | Jian-Ming Wang Ya-Zhi Fan Na Hui Lei Xiong Hai-Xiao Feng Nai-Xue Sun |
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| Institution: | Jian-Ming Wang,Ya-Zhi Fan,Na Hui,Lei Xiong,Hai-Xiao Feng,Nai-Xue Sun Department of Ophthalmology,the Second Affiliated Hospital of Medical College of Xi\'an Jiaotong University,Xi\'an 710004,Shaanxi Province,China |
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| Abstract: | AIM:To study the feasibility of recombinant adeno-associated virus(rAAV)as a vector to transfer the green fluorescent protein(GFP)gene as a target gene into rabbit retina.METHODS:Intravitreal injection of rAAV-gfp was performed in either eye for each rabbit with the other eye taken as control.At the 3rd,7th,and 14th day after injection,the eyeballs were removed,and the retinas were flat-mounted on glass slides to inspect the retinal fluorescence,respectively.RESULTS:After intravitreal injection of rAAV-gfp,the presence of fluorescent spots in the cytoplasm of retinal cells indicated that GFP gene was efficiently transferred and expressed in the rabbit retina.CONCLUSION:Recombinant adeno-associated virus is a reliable and simple vector for transferring target gene,e.g.,GFP gene,to the retina. |
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| Keywords: | recombinant adeno-associated virus:green fluorescent protein gene gene transfer retina |
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