The influence of preadsorbed canine von Willebrand factor, fibronectin and fibrinogen on ex vivo artificial surface-induced thrombosis |
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| Authors: | L K Lambrecht B R Young R E Stafford K Park R M Albrecht D F Mosher S L Cooper |
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| Institution: | 1. Department of Chemical Engineering, University of Wisconsin, Madison, WI, USA 53706;2. Department of Pharmacy, University of Wisconsin, Madison, WI, USA 53706;3. Department of Medicine, University of Wisconsin, Madison, WI, USA 53706;1. Department of Neurosurgery, Johns Hopkins School of Medicine, Baltimore, Maryland, USA;2. Department of Radiology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA;3. Department of Neurosurgery, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia;4. Department of Neurosurgery, Kennedy Krieger Institute, Kirby Research Center for Functional Brain Imaging, Baltimore, Maryland, USA;5. Department of Neurosurgery, Mayo Clinic Florida, Jacksonville, Florida, USA;6. Chimère EA, Research Team for Head & Neck, University of Picardie Jules Verne, Amiens, France;7. Department of Image Processing, Jules Verne University Hospital, Amiens, France;8. Department of Neurosurgery, Loyola University School of Medicine, Maywood, Illinois, USA;9. Department of Biomedical Engineering, Johns Hopkins School of Engineering, Baltimore, Maryland, USA;10. School of Medicine, Faculty of Health Sciences, Universidad de Santander UDES, Bucaramanga, Colombia;1. ITMO University, Saint Petersburg, Russian Federation;2. ITMO University, Saint Petersburg, Russian Federation;3. New York University School of Medicine, New York, the United States of America;1. Department of Orthopedic Surgery, Baylor College of Medicine, Houston, Tex;2. Department of Veterinary Medicine and Surgery, University of Texas MD Anderson Cancer Center, Houston, Tex;3. Department of Interventional Radiology, University of Texas MD Anderson Cancer Center, Houston, Tex;1. Department of Surgery, Beaumont Health, Royal Oak, Mich;2. Department of Radiology, Beaumont Health, Royal Oak, Mich;3. Section of Vascular Surgery, Beaumont Health, Royal Oak, Mich |
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| Abstract: | We have examined the effects of preadsorption of several canine plasma proteins on surface-induced thrombogenesis in a canine ex vivo model. Our technique allowed determination of initial deposition and subsequent embolization of 51Cr-labeled platelets and 125I-fibrinogen onto and from polymeric arterio-venous shunts in non-anticoagulated canines. Segments of the tubing were removed at various time points between 2 and 120 minutes of blood contact for examination of the morphology of the thrombus by scanning electron microscopy. Thrombus deposition was measured on uncoated plasticized poly(vinyl chloride) (PVC) and PVC precoated with canine von Willebrand factor (vWF), fibronectin, partially purified fibrinogen (fibrinogen which contained vWF and fibronectin as impurities), or purified fibrinogen (fibrinogen which had been further purified to remove fibronectin and vWF). Preadsorption of all proteins studied enhanced the thrombogenic response relative to that of the uncoated surface. Precoating with vWF or partially purified fibrinogen resulted in the deposition of the greatest number of thrombi, and embolization was slower than on shunts precoated with canine fibronectin or purified fibrinogen. The deposition-embolization profiles for the fibronectin and purified fibrinogen-coated surfaces were similar. The amount and time sequence of initial adhesion and spreading of platelets was related to the extent and time sequence of peak thrombus formation. The partially purified fibrinogen-coated and vWF-coated surfaces had more adhered and spread platelets at the earliest time points and a greater number of larger thrombi at the peak deposition times. The slowest rate of platelet adhesion and spreading was seen on the purified fibrinogen-coated surface. White blood cells were present very early on surfaces precoated with vWF and partially purified fibrinogen, and were present prior to embolization on all surfaces. Major conclusions from this work indicate that, although fibrinogen and fibronectin promote thrombogenesis when adsorbed to a surface, vWF is even more active in promoting platelet deposition and in anchoring thrombi to the surface of biomaterials. Thus, differences in vWF adsorption to biomaterials may be a determinant of surface-induced thrombogenesis. |
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