大鼠脊髓急性损伤后bax和bcl—2的表达

目的：检测大鼠脊髓损伤后凋亡相关基因的表达，以探讨神经细胞凋亡的分子机制。方法：大鼠脊髓（T8．9）经中度压迫损伤后，分别在30min、2h、4h、8h、24h、48h和72h处死取材（n=6)。主要应用免疫组化及原位杂交技术对脊髓组织进行标记，以检测bcl-2和bax的表达。结果：损伤4h后bax蛋白大量表达，而bcl-2蛋白仅有少量表达，bcl-2 mRNA未见表达。结论：脊髓损伤后凋亡基因bax大量表达，并可能在神经细胞的凋亡过程中起重要作用。

Expression of bax and bcl-2 after Acute Compression Injury to Rat Spinal Cord

We determined the expression of apoptosic correlative genes after spinal cord compression injury, to study the molecular mechanism of neuronal apoptosis. Methods: Following a controlled, moderate degree compression injury to the lower thoracic spinal cord (T8、9), rats were killed at 30min,2,4,8,24,48 or 72 hours after injury (n=6 per group). Three segments of every spinal cord were cut for morphological studies, including hematoxylin and eosin staining, Nissl staining, immunohistochemical staining and in situ hybridization methods. Results: Proteins Bax expressed at 4h after spinal cord injury. But Bcl-2 immunoreactivity was present in the lesion region with low expression, and bcl-2 mRNA without expression. Conclusion: There exist high expression of apoptosic correlative genes bax after spinal cord injury, it may play an important role in induction of neuronal cells to apoptosis.