| 体外细胞培养支原体的检测与清除 |
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| 引用本文： | 吴尚辉,彭聪,顾焕华,黄柏英,罗学滨,祝斌. 体外细胞培养支原体的检测与清除[J]. 中国现代医学杂志, 2004, 14(12): 111-113 |
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| 作者姓名： | 吴尚辉 彭聪 顾焕华 黄柏英 罗学滨 祝斌 |
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| 作者单位： | 中南大学湘雅医学院,细胞生物研究室,湖南,长沙,410078 |
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| 摘 要： | 目的检测及清除细胞培养中支原体的污染.方法应用DNA荧光法及两步PCR法检测支原体,采用BM-Cycline与裸鼠皮下过继联合法去除支原体;结果DNA荧光法、两步PCR法支原体检测率分别为25.0%,40.0%;BM-cyclie与裸鼠过继法对支原体的清除率为100.0%;结论上述方法能有效检测和清除细胞培养中支原体的污染.
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| 关 键 词： | 支原体 细胞培养 DNA荧光染色 两步PCR法 |
| 文章编号： | 1005-8982(2004)12-0111-03 |
To detect and remove the mycoplasma in vitro cell culture |
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| WU Shang-hui,PENG Cong,GU Huan-hua,HUANG Bai-yin,LUO Xue-bin,ZHU Bin. To detect and remove the mycoplasma in vitro cell culture[J]. China Journal of Modern Medicine, 2004, 14(12): 111-113 |
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| Authors: | WU Shang-hui PENG Cong GU Huan-hua HUANG Bai-yin LUO Xue-bin ZHU Bin |
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| Abstract: | Objective: To detect and remove mycoplasma containantion in cell culture. Methods: Detect mycoplasma by using DNA fluorescent and two-step PCR, removing mycoplasma by BM-Cycline and inoculation of nude mice. Results: The positive rates of mycoplasma by DNA fluorescent staining were 25.0%, The positive rates by two-step PCR were 40.0%; the removable rates of mycoplasma by BM-Cycline and inoculation of nude mice were 100.0%. Conclusions: Above methods can effectually detect and remove the mycoplasma in cell culture. |
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| Keywords: | mycoplasma cell culture DNA fluorescent staining two-step PCR |
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