| 4-氨基吡啶对牛磺酸调节突触体氨基酸释放的影响 |
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| 引用本文： | 郑里翔.4-氨基吡啶对牛磺酸调节突触体氨基酸释放的影响[J].药学学报,2001,36(4):307-309. |
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| 作者姓名： | 郑里翔 |
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| 作者单位： | 江西中医学院，江西南昌 330006 |
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| 摘 要： | AIM To elucidate the mechanism of taurine-regulated amino acid release from synaptosomes. METHODS Endogenous aspartate, glutamate and GABA release from cortical synaptosomes were measured by high performance liquid chromatography using stepwise elution system, Glutamate release was monitored by continuous fluorometry. RESULTS 4-Aminopyridine (3.0×10-2 mol*L-1) counteracted the taurine-induced inhibition of glutamate overflow (P<0.05), while aspartate and GABA release was not affected. Nimodipine (10-5 mol*L-1) combined with 4-aminopyridine was shown to decrease glutamate release (P<0.05). CONCLUSION Taurine may regulate glutamate release through presynaptic L-type calcium channel and aslo act on Asp-and GABA-nereve terminal to regulate Asp and GABA release in rat cortex.
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| 关 键 词： | 4-氨基吡啶 牛磺酸 突触体 谷氨酸 天冬氨酸 γ-氨基丁酸 尼莫地平 |
| 收稿时间： | 2000-05-13 |
EFFECT OF 4-AMINOPYRIDINE ON TAURINE-REGULATED RELEASE OF AMINO ACID FROM RAT CORTICAL SYNAPTOSOMES |
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| ZHENG Li-xiang.EFFECT OF 4-AMINOPYRIDINE ON TAURINE-REGULATED RELEASE OF AMINO ACID FROM RAT CORTICAL SYNAPTOSOMES[J].Acta Pharmaceutica Sinica,2001,36(4):307-309. |
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| Authors: | ZHENG Li-xiang |
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| Institution: | Jiangxi College of Chinese Traditional Medicine, Nanchang 330006, China. |
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| Abstract: | AIM: To elucidate the mechanism of taurine-regulated amino acid release from synaptosomes. METHODS: Endogenous aspartate, glutamate and GABA release from cortical synaptosomes were measured by high performance liquid chromatography using stepwise elution system, Glutamate release was monitored by continuous fluorometry. RESULTS: 4-Aminopyridine (3.0 x 10(-2) mol.L-1) counteracted the taurine-induced inhibition of glutamate overflow (P < 0.05), while aspartate and GABA release was not affected. Nimodipine (10(-5) mol.L-1) combined with 4-aminopyridine was shown to decrease glutamate release (P < 0.05). CONCLUSION: Taurine may regulate glutamate release through presynaptic L-type calcium channel and also act on Asp- and GABA-nereve terminal to regulate Asp and GABA release in rat cortex. |
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| Keywords: | taurine synaptosome glutamate aspartate GABA nimodipine 4-aminopyridine |
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