| CO_2对人卵巢癌细胞SKOV3转移因子表达的影响 |
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| 引用本文： | 黄金,唐均英,姚珍薇. CO_2对人卵巢癌细胞SKOV3转移因子表达的影响[J]. 现代妇产科进展, 2007, 16(1): 29-32 |
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| 作者姓名： | 黄金 唐均英 姚珍薇 |
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| 作者单位： | 重庆医科大学附属第一医院妇产科,重庆,400016 |
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| 摘 要： | 目的:探讨二氧化碳(CO_2)对卵巢恶性肿瘤细胞转移能力的影响。方法:体外培养人卵巢浆液性乳头状腺癌SKOV3细胞株,分为CO_2组、N_2组和对照组,分别在CO_2及N_2处理后2h,置于常规条件下培养12、24、48h通过RT-PCR和Western Blot检测不同时相血管内皮生长因子C(VEGF-C)和基质金属蛋白酶9(MMP9)的表达。结果:CO_2处理后,SKOV3细胞VEGF-C和MMP9的mRNA和蛋白质表达显著增加(P＜0．05);N_2处理后,SKOV3细胞VEGF-C和MMP9的mRNA和蛋白质表达较对照组增加(P＜0．05)。结论:CO_2促进了SKOV3 VEGF-C和MMP9的表达,对SKOV3的转移能力有增强作用。
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| 关 键 词： | 卵巢肿瘤 二氧化碳 细胞株SKOV3 血管内皮生长因子-C 基质金属蛋白酶-9 |
| 文章编号： | 1004-7379（2007）01-0029-04 |
| 收稿时间： | 2006-07-02 |
| 修稿时间： | 2006-07-02 |
Effects of carbon dioxide on the expression of metastatic factors in human ovarian carcinoma cell line SKOV3 |
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| Huang Jin,Tang Junying,Yao Zhenwei. Effects of carbon dioxide on the expression of metastatic factors in human ovarian carcinoma cell line SKOV3[J]. Current Advances In Obstetrics and Gynecology, 2007, 16(1): 29-32 |
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| Authors: | Huang Jin Tang Junying Yao Zhenwei |
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| Affiliation: | Department of Obstetrics and Gynecology,First Affiliated Hospital of Chongqing University of Medical Sciences,Chongqing 400016 |
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| Abstract: | Objective:To study the effects of carbon dioxide on the expression of meta- static factors in ovarian carcinoma cell line SKOV3.Method:Ovarian carcinoma cell line SK- OV3 was treated with 100% carbon dioxide for 2 hours,then put into normal circumstance for 12hours,24hours,48hours,respectively;Examine the expressions of different times of VEGF-C and MMP9 by RT-PCR and Western Blot.Result:After the treatment of carbon dioxide,the ex- pression of VEGF-C and MMP9 significantly increased compared with controlled groups.Con- clusion:Carbon dioxide promotes the expression of VEGF-C and MMP9 in SKOV3,so it may improve the metastatic capability of ovarian carcinoma cell line. |
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| Keywords: | Ovarian neoplasms Carbon dioxide Cell line SKOV3 Vascular endothelial growth factor-C Matrix metalloproteinase-9 |
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