| sFGFR1基因的克隆与在RTS系统中的高效表达 |
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| 引用本文： | 陈勇,崔大祥,孙凯,杨雁灵,戴辉. sFGFR1基因的克隆与在RTS系统中的高效表达[J]. 医学争鸣, 2003, 24(8): 703-705 |
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| 作者姓名： | 陈勇 崔大祥 孙凯 杨雁灵 戴辉 |
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| 作者单位： | 1. 第四军医大学西京医院肝胆外科,陕西,西安,710033
2. 第四军医大学科研部基因诊断技术研究所,陕西,西安,710033 |
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| 基金项目： | 国家自然科学基金资助项目 (30 0 70 2 1 0 ) |
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| 摘 要： | 目的：克隆sFGFRl(soluble fibroblast growth factors receptor-1)基因，并在RTS(rapid translation system)系统中高效表达相应蛋白．方法：培养Swiss rat 3T3 fibroblast细胞株，提取总RNA，用RT—PCR方法获取鼠sFGFR1 cDNA片段，酶切后克隆到pIVEX2．3d载体并进行序列分析；采用Roche RTS ProteinMaster500系统，高效表达sFGFR1蛋白并用Western Blot鉴定表达的蛋白．结果：克隆了sFGFR1基因，测序证实序列正确；Western Blot证实sFGFR1蛋白在RTS系统中高效表达．结论：克隆了sFGFR1基因并在RTS系统获得高效表达．
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| 关 键 词： | 受体 成纤维细胞生长因子 克隆 分子 RTS 基因表达 |
| 文章编号： | 1000-2790(2003)08-0703-03 |
| 修稿时间： | 2002-09-28 |
Cloning of sFGFR1 and its high efficient expression in RTS |
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| Abstract: | AIM: To clone sFGFR1 gene and express corresponding protein in RTS. METHODS: Swiss rat 3T3 fibroblasts were cultured, total RNAs were extracted and sFGFR1 cDNA was obtained by RT PCR. The fragments were cut by means of NcoI and SmaI and products were cloned into pIVEX2 3 d vector and sequenced. SFGFR1 protein was expressed by using RTS ProteinMaster 500 and products were analyzed by Western Blot. RESULTS: sFGFR1 gene was cloned and sequencing analysis confirmed the cloned sequence was correct. Western Blot results confirmed sFGFR1 was highly expressed. CONCLUSION: sFGFR1 gene is cloned and highly expressed in RTS. |
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| Keywords: | receptors fibroblast growth factor cloning molecular rapid translation system gene expression |
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