正己烷致大鼠过氧化损伤及肝细胞凋亡的实验研究

目的研究正己烷(n-hexane)对大鼠的脂质过氧化损伤和肝细胞凋亡的影响。方法40只雄性SD大鼠随机分成5组,即阴性对照组、染毒组(75、150、300 mg/kg)和阳性对照组(环磷酰胺),每组8只。经腹腔注射染毒4周后,检测肝组织匀浆超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力,血清还原型谷胱甘肽(GSH)和丙二醛(MDA)含量;用流式细胞仪检测肝细胞凋亡情况。结果随着染毒剂量的增加,肝组织匀浆中SOD、GSH-Px活力、血清GSH含量降低,而血清MDA含量增大,组间比较差异有统计学意义(P<0.05或P<0.01),流式细胞仪检测结果显示,肝细胞凋亡率(AV /PI-)组间比较差异有统计学意义(P<0.01),与染毒剂量作相关分析,相关系数为0.913,无明显的相关性(P>0.05)。结论正己烷可引起或增强机体氧自由基反应,导致脂质过氧化损伤,引起肝细胞凋亡和坏死。

Experimental study on the effect of n-hexane on lipid peroxidation damage and hepatic cell apoptosis in rats

Objective To study the effect of n-hexane on lipid peroxidation damage and hepatic cell apoptosis in rats.Method 40 SD male rats were randomly divided into 5 groups,namely negative control group,75 mg/kg n-hexane group,150 mg/kg n-hexane group,300 mg/kg n-hexane group and positive control group(cyclophosphamide),8 rats for each group.Administrations had been given by intraperitoneal injection for four weeks,then the activities of GSH-Px and SOD in hepatic homogenate and the levels of GSH and MDA in serum apoptosis rate of the hepatic cell were detected,respectively.Result The results showed that with the increasing of n-hexane dosage,the activities of GSH-Px and SOD in hepatic homogenate and the concentration level of GSH in serum were reduced,while the serum MDA level was increased,there were statistic significances among the groups(P<0.05 or P<0.01).The detection of hepatocytic apoptosis rate by FCM showed that there were also some differences existed among the groups,but no correlation between dosage and apoptosis rate was found(r=0.913,P>0.05).Conclusion n-Hexane could induce or enhance the lipid peroxdation damage,hepatocytic apoptosis or necrosis in rats.