诱导剂在肺癌患者染色体不稳定性检测中的作用

作者检测了３０例肺癌患者（包括腺癌、鳞癌、小细胞瘤各１０例）和２０例正常对照个体自发和阿糖胞苷诱发的外周血淋巴细胞染色体畸变率和脆性位点表达率。对腺癌、鳞癌和小细胞癌患者作这两种指标的比较，结果显示在自发状态下，三种类型的患者之间无显著性差异；而阿糖胞苷诱导后则表现出腺癌和其它两类癌患者之间的显著性差异。说明自发状态下，染色体的不稳定性没有得到充分体现，而诱导剂阿糖胞苷的应用起到了＂放大＂作用，使染色体不稳定性得到充分体现，差别距离拉大而易于观察判断。同时，作者对如何确定诱导剂的浓度及染色体的稳定性等问题进行了讨论。

THE FUNCTION OF INDUCER IN DETECTING CHROMOSOME INSTABILITY OF THE PATIENTS WITH LUNG CANCER

Chromosome aberration rate and fragile site expressive frequency of peripheral lymphocytes were detected in 20 healthy individuals and 30 patients with three types of lung cancer including adenocarcinoma , squamous cell cancer and small cell cancer. The result showed that no significant difference in spontaneous chromosome aberration rate and fragile site expressive frequency was found among the three types of lung cancer patients, but a significant difference was displayed as induced with Ara-C. It demonstrated that: (1) the chromosome instability was not displayed spontaneously; (2) when induced with Ara-C,the chromosome instability among the three types increased. The chromosome stability and the selection of inducer' s dosage are discussed