肺癌鲎凝集素受体的研究

应用胶体金标记鲎凝集素（Ｌｉｍｕｌｕｓｐｏｌｙｐｈｅｍｕｓａｇｇｌｕｔｉｎｉｎ，ＬＰＡ）标记４例正常肺组织和５４例肺癌组织，各例还用生物素化蓖麻（ＲＣＡ）、菜豆（ＰＨＡ）、双花藕豆（ＤＢＡ）和麦胚凝集（ＷＧＡ）作为对照标记。结果表明：１．ＬＰＡ受体在正常肺组织中选择性分布在大支气管上皮杯状细胞和粘膜下浆液性细胞，在非小细胞癌中的阳性率为５７．１％（２０／３５），小细胞癌、类癌中的阳性率为６３．２％（１２／１９）；２．与ＲＣＡ、ＰＨＡ、ＤＢＡ和ＷＧＡ受体表达相比，ＬＰＡ受体表达可能与肺癌发生的关系更密切，ＬＰＡ受体在肺癌中的阳性率较ＲＣＡ、ＰＨＡ、ＤＢＡ和ＷＧＡ受体表达阳性率高，而且在小细胞癌和类癌中阳性率相差均有显著性意义（Ｐ＜０．０５）。

LIMULUS POLYPHEMUS LECTIN RECEPTOR IN LUNG CARCINOMA

Limulus polyphemus agglutinin(LPA) has an affinity for glycoproteins(receptor) containing N-glycolyl or N-acetylneuraminic residues. Four cases of normal lung tissue and fifty-four cases of lung carcinoma were studied with colloid gold labeled LPA by using direct histochemical method.The results revealed that:In normal lung tissue, LPA receptor was selectively stained within the epithelial goblet cells and the serous cells of submucosal glands. The expression of LPA receptor was more frequently seen in some types of lung carcinoma than that of RCA, PHA, DBA and WGA receptors(P<0.05), and was the same as in carcinoid and small-cell lung carcinoma(P<0.05).The study suggests that histogenesis of lung carcinoma is related to the change of the expression of LPA receptor.