血管紧张素Ⅰ转换酶基因多态性与2型糖尿病肾病的关联性

目的:探讨血管紧张素I转换酶(ACE)基因多态性与2型糖尿病肾病的关系。方法:2型糖尿病并发肾病组(DN)患者80例,单纯2型糖尿病组(DM)患者60例,60名健康人作对照(正常对照组),抽提每人外周血中白细胞基因组DNA,采用聚合酶链反应(PCR)扩增ACE基因第16内含子的一个287bp Alu的插入/缺失(I/D)基因片段,进行1.5%的琼脂糖凝胶电泳,并在紫外线灯下观察荧光带并确认每例的基因型。各组间的基因型频数分布应用Hardy-Weinberg遗传平衡定律检验和χ2检验。等位基因频数分布应用χ2检验。结果:ACE基因的3种基因型Ⅱ、ID和DD的频数分布符合Hardy-Weinberg平衡定律;各组间ACE基因的基因型频数分布比较差异无显著性(χ2=1.10,P>0.05);3组的等位基因I和D的频数分布比较差异无显著性(χ2=0.84,P>0.05)。结论:ACE基因多态性与2型糖尿病肾病的发病无关联性。

Association between ACE gene polymorphism and type 2 diabetic nephropathy

Objective To investigate the association between angiotensin Ⅰ converting enzyme(ACE) gene polymorphism and type 2 diabetic nephropathy.Methods In 80 patients with diabetic nephropathy(DN),60 patients with type 2 diabetes mellitus (DM)and 60 health persons (control),the white cell template DNA of peripheral blood were extracted,the ACE gene (the 16th intron of 287 bp I/D) polymorphism was determined by polymerase chain reaction (PCR),then 1.5% agarose gel electrophoresis was performed,the results were observed under ultraviolet light,the genotypes of each case were confirmed,the genotypic frequency and allelic frequency of ACE gene were compared between three groups with Hardy-Weinberg inherit quilibrium laws and Chi-square criterion.Results The distribution of genotypic frequencies of ACE was not deviated from the Hardy-Weinberg equilibrium;there were no remarkable differences of the frequency distributions of I I,ID and DD of ACE between three groups (χ2 =1.10,P>0.05),and the frequency distributions of I and D had no markedly differences between three groups(χ2 =0.84,P>0.05).Conclusion There is no significant correlation between ACE gene polymorphism and type 2 diabetic nephropathy.