Antiinflammatory effects of Viola tricolor gel in a model of sunburn in rats and the gel stability study |
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Authors: | Mariana Piana Mariane Arnoldi Silva Gabriela Trevisan Thiele Faccim de Brum Cássia Regina Silva Aline Augusti Boligon Sara Marchesan Oliveira Marina Zadra Carin Hoffmeister Mateus Fortes Rossato Raquel Tonello Luciane Varini Laporta Robson Borba de Freitas Bianca Vargas Belke Roberta da Silva Jesus Juliano Ferreira Margareth Linde Athayde |
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Institution: | 1. Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Avenida Roraima 1000, Camobi, Santa Maria, RS, Brazil.;2. Graduate Program in Biological Sciences: Toxicological Biochemistry, Brazil;3. Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil;4. Laboratory Quality Control of Drugs, Franciscan University Center, Build 3, room S011, 97010-032, Santa Maria, RS, Brazil |
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Abstract: | Ethnopharmacological relevanceViola tricolor, popularly known as heartsease has been empirically used in several skin disorders, including burns.Aim of the studyThe objective of this study was investigate the antinociceptive and antiinflammatory effect of a gel containing extract of Viola tricolor flowers on thermal burn induced by UVB irradiation and to perform gel stability study.MethodsThe antinociceptive and antiinflammatory effect were evaluated by static and dynamic mechanical allodynia model, paw edema, and neutrophilic cell infiltration. Metabolites compounds were quantified by HPLC. The gel stability study was performed analyzing organoleptical aspects, besides pH, viscosity, and quantification of rutin by HPLC.ResultsIn the results were evidenced changes in threshold in statical and dynamic mechanical allodynia (Imax=100±10% and 49±10%, respectively), paw edema (Imax=61±6%), and myeloperoxidase activity (Imax=89±5%). Such effects may be attributed, in part, to rutin, salicylic and chlorogenic acids, and others compounds found in this species. No important changes were detected in the stability study, in all aspects analyzed in temperature below 25 °C.ConclusionThese findings suggest that Viola tricolor gel has an antinociceptive and antiinflammatory effect in the ultraviolet-B-induced burn, since maintain the temperature below 25 °C. |
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Keywords: | Viola tricolor Antiinflammatory Antinociceptive Gel stability Violaceae |
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