Chemical composition and antinociceptive,anti-inflammatory and antiviral activities of Gallesia gorazema (Phytolaccaceae), a potential candidate for novel anti-herpetic phytomedicines |
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Authors: | Ademir de Jesus Silva Júnior Fátima de Campos-Buzzi Maria Teresa Villela Romanos Theodoro Marcel Wagner Antônio Fernando de Paula Costa Guimarães Valdir Cechinel Filho Ronan Batista |
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Institution: | 1. Departamento de Estudos Básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, 45700-000 Itapetinga, BA, Brazil;2. Programa de Pós-Graduação em Ciências Farmacêuticas e Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí, Rua Uruguai, 458, 88302-202 Itajaí, SC, Brazil;3. Laboratório Experimental de Drogas Antivirais e Citotóxicas, Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS—Bl. I, Ilha do Fundão, Cidade Universitária, 21.941-590 Rio de Janeiro, RJ, Brazil;4. Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Geremoabo, s/n, Ondina, 40170-290 Salvador, BA, Brazil |
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Abstract: | Ethnopharmacological relevanceIn traditional medicine, teas made from leaves and bark of Gallesia gorazema are used as antispasmodic, anthelmintic, antihemorrhagic and febrifuge agents. Crude leaves of this plant are also employed as a remedy in the treatment of abscesses, orchitis, gonorrhea and for rheumatic pain relief. this study investigates the presumed antinociceptive and anti-inflammatory activities of leaves and roots Gallesia gorazema (Phytolaccaceae) extracts. The most active extract and its isolated compound, a new natural product, are also evaluated against viruses HSV-1 and HSV-2.Materials and methodsIn vivo experiments with mice were used to assess the analgesic and anti-inflammatory activities of Gallesia gorazema extracts. Antiviral activity of extracts and the new natural product was investigated by in vitro experiments.ResultsResults show that dichloromethanic root (DRE) and ethanolic leaf (ELE) extracts displayed significant antinociceptive and anti-inflammatory activities in in vivo experiments with mice. Both extracts were also assayed against the herpes simplex viruses HSV-1 and HSV-2, but only DRE was highly active, showing a selective antiviral effect against HSV-1. Phytochemical fractionation of DRE led to the isolation of 28-hydroxyoctacosyl ferulate, a novel natural product, which displayed strong antiviral activity against HSV-1 (EC50=21.6 μg/mL) with a selective index above 9, justifying, at least in part, the high selective antiviral activity observed for DRE.ConclusionThese results suggest that the plant Gallesia gorazema is a potential candidate for the development of novel anti-herpetic phytomedicines. |
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Keywords: | Gallesia gorazema Antinociceptive and anti-inflammatory activities Herpes Antiviral activity Anti-herpetic phytomedicines 28-Hydroxyoctacosyl ferulate |
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