心经经脉与心脏相关的差异表达基因的研究

目的:从基因差异表达方面探讨心经与心脏相关的特异性基础。方法:复制大鼠急性心肌缺血模型,分别电针心经“神门”至“通里”段、小肠经“养老”至“支正”段、肺经“太渊”至“列缺”段。刺激电压5V,频率2Hz,波宽300μs,正负向交替方波,每次电刺激20min,1次/d,共3次。取缺血心肌组织,采用大鼠全基因U230序列芯片进行芯片检测,大规模筛选差异表达基因。结果:与模型组比较,差异表达2倍以上的基因,电针心经组有20个上调70个下调;电针小肠经组有18个上调26个下调;电针肺经组有14个上调20个下调,且3组之间鲜有相同的基因表达。结论:经脉脏腑相关具有确实的分子基础,电针心经、小肠经、肺经具有相对特异性作用途径。

Research on the Differentially Expressed Genes Related to the Relatively Specific Connection Between the Heart Meridian and the Heart

OBJECTIVE: To study the scientific basis of specific correlation between the meridian and the Zang- or Fu organ by using gene differential expression (gene-chip technique) in the rat. METHODS: Twenty-one SD rats were randomly and evenly divided into control group, sham-myocardial ischemia (MI) group, MI group, electroacupuncture (EA)-Heart-Meridian (HT) group, EA-Lung-Meridian (LU) group and EA-Small-Intestine-Meridian (SI) group. EA (frequency 2 Hz, stimulating strength 5 V, pulse duration 300 mus) was applied to "Shenmen" (HT 7)- "Tongli" (HT 5) (Heart Meridian), "Y anglao" (SI 6)- "Zhizheng" (SI 7) (Small Intestine Meridian), "Taiyuan" (LU 9)-"Lieque" (LU 7) (Lung Meridian) for 20 min, once daily and continuously for 3 days. Myocardial ischemia model was duplicated by occlusion of the anterior descending branch of the left coronary artery, and myocardial tissue of the left ventricle was taken 3 days after EA treatment for analyzing the differentially expressed genes in different groups with rat whole gene U 230 sequence chips (offered by Affymetrix Company). RESULTS: Compared with model group, among the differentially expressed genes being twofolds and above, the up-regulated and the down-regulated were 20 and 70 in EA-HT group, 18 and 26 in EA-SI group, and 14 and 20 in EA-LU group separately. Few same genes with the same differential expression were found among the 3 EA groups. CONCLUSION: There is exactly a molecular basis in the correlation between Heart Meridian and the heart, and EA of Heart Meridian, Small Intestine Meridian and Lung Meridian have their own special signaling pathways.