组胺对T细胞IL-2产生及增殖活性影响的实验研究

目的:了解组胺对 CD4 和 CD8 T细胞IL-2产生和细胞增殖活性的影响。方法:密度梯度离心及吸附法分离PBMC和PBLC,采用抗CD4 和CD8 抗体分别制备CD8 和CD4 T细胞进行培养,然后采用ELISA法和MTT比色法测上清液IL-2含量及增殖活性。结果:①组胺 CD4 （CD8 ）培养上清液中IL-2水平及MTT增殖指数与T细胞自然培养孔比较明显降低（P<0.05）。②组胺 CD4 （CD8 ） 西咪替丁培养孔上清液中IL-2水平及 MTT增殖指数明显高于未加西咪替丁孔（P<0.05）。③CD4 T细胞自然培养孔上清液中IL-2水平显著高于CD8 T细胞自然培养孔。结论:组胺可抑制T细胞IL-2产生及增殖。西咪替丁可阻断组胺对T细胞的抑制作用。CD8 T细胞也可产生IL-2,但其功能较 CD4 T细胞为低。

Influence of histamine on interleukin-2 production and proliferation of T lymphocytes in vitro

Objective:To investigate the influence of histamine on intedeukin(IL-2) production and proliferation of CD4 T lymphocytes and CD8 T lymphocytes.Methods:Density gradient centrifugalization and absorption technique were respecitively applied to detach peripheral blood mononuclear cells(PBMC) and peripheral blood lymphocytes(PBLC). CD4 and CD8 T lymphocytes divided with anti-CD4 and anti-CD8 antibody were cultured in vitro. IL-2 levels in supernatant were quantified by enzyme-linked immunosobent assay (ELBA), and proliferation index were measured by MTT methods. Results:IL-2 levels in supernatant and proliferation rate of CD4 and CD8 T lymphocytes treated with histamine were significantly lower than those of their corresponding un-stimulated controls(P<0.05). IL-2 production and proliferation rate of CD4 (CD8 ) T lymphocytes co-cultured with histamine and cimetidine were higher than those of the controls cultured with histamine alone( P < 0.05). CD4 T lymphocyte produced much more IL-2 than CDS* . Conclusion: Histamine inhibit T lymphocytes proliferation and IL-2 production,and the inhibitory effect can be stocked by cimetidine.CDS* T lymphocytes also secret IL-2,but less than CD4 T cell.