6种黄酮类化合物拮抗肝细胞毒性作用的比较

目的观察芹菜素、木犀草素、白杨素、高良姜素、山奈酚、黄芩素6种结构相近的黄酮类化合物对肝毒性物质诱导的人正常肝细胞株L-02细胞及小鼠原代肝细胞损伤的保护作用,并对其保护肝细胞损伤活性的强弱进行比较及结构分析。方法培养的L-02细胞及小鼠原代肝细胞分别给予10mmol·L~(-1)四氯化碳(CCl_4)、500μmol·L~(-1)牛磺酸脱氧胆酸(TDCA)、2μmol·L~(-1)川楝素(toosendanin,TSN)和25μmol·L~(-1)千里光碱(senecionine,SENE)诱导细胞凋亡。6种黄酮类化合物(100μmol·L~(-1))分别与细胞预孵15min后,加入肝毒性物质,48h后MTT法检测细胞存活率。结果与空白对照组相比,4种肝毒性药物都能明显降低肝细胞的存活率(P<0.01)。黄酮类化合物处理后,黄芩素和山奈酚能不同程度地提高肝细胞损伤模型组的细胞存活率(P<0.01)。其他4种黄酮类化合物对肝细胞存活率无显著作用。结论黄芩素和山奈酚具有抑制肝毒性物质诱导的L-02细胞和小鼠原代肝细胞毒性的作用,有潜在的抗氧化保肝活性,而芹菜素、木犀草素、白杨素、高良姜素无明显的拮抗肝毒性作用。

Comparison in protective effects of six flavonoids against hepatotoxins-induced cytotoxicity

AIM To observe the protective effects of apigenin,luteolin,chrysin,galangin,kaemp-ferol and baicalein present study is designed of six flavonoids with similar structure against hepatotoxicity in human L-02 cells and mouse primary hepatocytes,and explore the relationship between their structures and actions.METHODS The cultured human L-02 cells and mouse primary hepatocytes were treated with carbon tetrachloride(CC1_4,10mmol·L~(-1)),toosendanin(TSN,2μmol·L~(-1)),senecionine(SENE,25μmo·L~(-1)) and deoxycholic acid taurine(TDCA,500μmol·L~(-1)),respectively.The hepatotoxins were added after flavonoids(100μmol·L~(-1)) were preincubated with the cells for 15 min.MTT assay was used to detect the cell viability after the incubation for 48 h.RESULTS Four hepatotoxins could siginificantly reduce the cell viability (P<0.01) compared with the control group.After the treatments,baicalein and kaempferol could increase the cell viability of hepatotoxins-treated hepatocytes(P<0.01).However,other compounds had no protective effects against hepatotoxicity.CONCLUSION Baicalein and kaempferol could both significantly prevent these hepatotoxins-induced cytotoxicity in human L-02 cells and mouse primary hepatocytes,however,apigenin, luteolin,chrysin and galangin failed to do so.