桂哌齐特拮抗氧化低密度脂蛋白对人脐静脉内皮细胞的损伤

目的研究桂哌齐特对氧化低密度脂蛋白(ox-LDL)损伤人脐静脉内皮细胞系ECV304的保护作用。方法传代培养ECV304细胞,随机分为空白对照组、模型组、桂哌齐特(200mg·L~(-1)和400mg·L~(-1))组,与ox-LDL共同孵育24h制模,观察培养上清液中一氧化氮(NO)与丙二醛(MDA)含量的变化,测定Fura-2/AM负载后的内皮细胞的游离静息钙浓度(Ca~(2+)]_i)。结果 ox-LDL诱导的模型组上清液中NO的浓度明显降低,MDA含量显著升高(均P<0.01)。桂哌齐特组NO浓度增加(P<0.01),MDA含量降低均(P<0.05)。与空白对照组相比,模型组的Ca~(2+)]_i显著升高,桂哌齐特组能降低损伤导致的Ca~(2+)]_i升高(均P<0.01)。结论桂哌齐特能拮抗ox-LDL对人脐静脉内皮细胞的损伤作用。

Protective effects of cinepazide against injury by oxidized low-density lipoprotein in ECV304 cells

AIM To study the protective effects of cinepazide on ECV304 cells injury by oxidized low-density lipoprotein(ox-LDL).METHODS ECV304 cells were cultured and divided into control group,model group and cinepazide groups(200 mg·L~(-1) and 400 mg·L~(-1)).The model group and cinepazide groups were incubated with the ox-LDL for 24 h.Then the concentrations of NO and MDA in the supernatant of medium were tested.Using Fura-2/AM as fluorescent Ca~(2+) indicator,the free cytosolic calcium(Ca~(2+)]_i) was measured. RESULTS Ox-LDL decreased the concentration of NO in medium,while increased the concentration of MDA significantly than the control group(P<0.01).Fura-2/AM loaded ECV304 cells with ox-LDL exhibited higher Ca~(2+)]_i than that from the control group(P<0.01),while the cinepazide groups reversed the effect of ox-LDL (P<0.05).CONCLUSION Cinepazide could protect ECV304 cells against ox-LDL-induced injury.