多重PCR检测婴幼儿腹泻样本中常见病毒

目的初步了解深圳地区引起临床婴幼儿急性腹泻中常见病毒的病原分布情况,为临床防治提供依据。方法采用多重RT-PCR方法检测婴幼儿急性腹泻常见病毒:轮状病毒(RV)、星状病毒(AstV)、诺如病毒(NV),采用单重PCR法筛查临床腹泻标本肠道腺病毒(EAdV)。结果在440份标本中,共检出197例至少感染一种腹泻病毒,总阳性率为44.77%(197/440),其中183例为一种病毒感染,14例(3.18%)为混合感染。4种腹泻病毒以RV感染为主,阳性率为28.64%(126/440);其次是EAdV,阳性率为8.86%(39/440);NV阳性率为5.45%(24/440);AstV阳性率为1.82%(8/440)。混合感染以RV合并其他病毒感染为主,占92.86%。结论 RV、EAdV和NV是引起深圳地区婴幼儿急性腹泻的主要病毒致病原,混合感染以RV联合其他病毒感染为主,临床上应根据所感染病原体制定有针对性的诊治措施。

Multiplex PCR to identify infant diarrhea common virus

Objective Preliminary understanding of the common viral pathogen distribution which caused the clinical infant acute diarrhea in Shenzhen; Providing the basis information support for clinical prevention and treatment. Methods Multiplex RT-PCR method for detection common virus: RV, AstV; NV, which caused acute diarrhea in infants. Clinical diarrhea specimens EAdV were screened by single PCR method. Results In the 440 specimens, 197 cases were detected at least a diarrhea virus infection, the total detection rate was 44.77% (197 / 440), of which 183 cases of a viral infection, 14 cases (3.18%) were mixed infections. Four kinds of diarrhea virus infection were RV 28.64% (126 / 440), EAdV 8.86% (39 / 440), NV 5.45% (24 / 440) and AstV 1.82% (8 / 440). Mixed infection with RV in combination with other viral infections was accounting for 92.86%. Conclusion RV, EAdV and NV are the major viral pathogens which caused the acute diarrhea in infants in Shenzhen, mixed infections with RV infection mainly in conjunction with other clinical pathogens. Clinical treatments should be based on the viral pathogens.