A technique for quantitative cytology of nasal secretions |
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| Authors: | H S Lee Y Majima Y Sakakura B W Kim |
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| Institution: | (1) Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, 514 Tsu, Mie, Japan;(2) Department of Otolaryngology, Catholic University Medical College, 62 Yoido-dong, 150-010 Yongdungpo-ku, Seoul, Korea |
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| Abstract: | Summary To quantitatively analyze cellular elements in mucopurulent nasal secretions from patients with chronic paranasal sinusitis, we studied five mucus-liquefying agents — dithiothreitol (DTT), ethylenediaminetetraacetic acid tetrasodium salt, propylene glycol, serratio peptidase and urea — with Hanks' balanced salt solution used as a control. Agents were compared for the number of cells, the mucus-liquefying effect and the staining characteristic of cells in cytocentrifuge-prepared smears. The results indicated that the treatment of nasal secretions with 10 mM DTT provides more clear and detailed cytological preparations for quantitative cytology of nasal secretions. |
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| Keywords: | Quantitative cytology Nasal secretions Dithiothreitol Chronic paranasal sinusitis |
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