bBMP对体外培养人胎儿关节软骨细胞分化的促进作用

为了解运动创伤中肌肉、肌腱、关节囊中异位化骨的机制及软骨细胞在此过程中所起的作用，本研究用牛骨形态发生蛋白（ｂＢＭＰ）诱导体外不传代培养的胎儿关节软骨细胞，发现：在ｂＢＭＰ的诱导下，不传代培养的软骨细胞逐渐变为成骨细胞样形态，它们表达的碱性磷酸酶明显增加，细胞周围的钙沉积和基质沉积也明显增加。在ｂＢＭＰ的诱导下，关节软骨细胞开始表达具有成骨细胞特异性和骨基质特异性的骨钙素。这些结果表明，在ｂＢＭＰ的诱导下，胎儿关节软骨细胞已开始表达成骨细胞表型，并向成骨细胞样细胞方向分化。本研究提示，在异位化骨和各种软骨内化骨过程中，软骨细胞可能具有较活跃的参与骨形成的作用，可能不像以前所假设的那样仅分化为肥大软骨细胞，然后变性，最终坏死。

Effect of bBMP on Promoting the Differentiation of Human Embryo Articular Chondrocytes Cultured in Vitro

To understand the pathogenesis of ectopic osteogenesis in muscle,ligament and articular capsule and the action of chondrocytes in these endochondral osteogenesis,bovine bone morphogenetic protein(bBMP)was used to induce embryo articular chond rocytes cultured in vitro,The results showed that under the induction of bBMP primary,cultured chondrocytes gradually changed their shapes into osteoblast-like cells,the expression of alkaline phosphatase of these cells increased obviously,and the calcium and matrix deposits arround the cultured cells were also increased remarkably,Under the induction of bBMP, articular chondrocytes began to express osteocalcin which was a spec ific marker for osteoblasts and bone matrix,These results showed that embryo articu lar cbondrocytes had begun to express the phenotypes of osteoblasts and differentiated to osteoblast-like cells under the influence of bBMP. It was suggested that, chondroc ytes might be able to play an active and important part in ectopic bone formatian and other kinds of endochondral osteogenesis, but not simply differentiated towards hypertrophic chondrocytes and then degenerated,and necrosed in the end.