应用FRTL-5细胞探索五氯苯酚干扰甲状腺激素作用机制

目的利用FRTL-5细胞,在体外研究五氯苯酚干扰甲状腺激素活性的机制。方法设二甲亚砜溶剂对照组(DMSO);高氯酸钠摄碘阳性对照组;五氯苯酚:0.1μg/ml、0.3μg/ml和0.5μg/ml三个剂量组;五氯苯酚处理FRTL-5细胞24小时,用3H掺入法测其对FRTL-5细胞DNA合成的影响,放免法测其对培养液中甲状腺球蛋白浓度的影响;用五氯苯酚、高氯酸钠处理FRTL-5细胞12和24小时后,用同位素125I测摄碘能力的变化。结果3H掺入法显示五氯苯酚各剂量组对FRTL-5细胞DNA合成无显著影响;放免法显示五氯苯酚0.3μg/ml和0.5μg/ml剂量组显著降低甲状腺球蛋白浓度;五氯苯酚处理FRTL-5细胞12小时后,0.3μg/ml和0.5μg/ml剂量组使FRTL-5细胞摄碘能力显著增强,处理24小时后,FRTL-5细胞摄碘能力无显著改变,但有降低的趋势,高氯酸钠阳性对照组使FRTL-5细胞摄碘能力在12小时和24小时都极显著降低。结论五氯苯酚干扰甲状腺激素作用可能与其使甲状腺球蛋白浓度的改变有关。

Study of the mechanism of thyroid hormone disrupted by pentachlorophenol in FRTL-5 cell

OBJECTIVE: To study the mechanism of thyroid hormone action disrupted mechanism by pentachlorophenol in FRTL-5 cells. METHODS: In the study solvent control group (DMSO), positive control group in iodide uptake measurements (perchlorate sodium) and three pentachlorophenol groups were designed. FRTL-5 cells were treated at the doses of 0.1 microg/ml, 0.3 microg/ml and 0.5 microg/ml of pentachlorophenol for 24 hours. The proliferation of FRTL-5 cells was assayed by 3H - TdR, and the concentrations of thyroglobulin in culture were assayed by RIA. In iodide uptake measurements, and the 125I uptake of FRTL-5 cells was tested in 12 h and 24 h. RESULTS: Pentachlorophenol had no effect on the proliferation of FRTL-5 cells, and the concentrations of thyroglobulin in culture were decreased at the levels of group 0.3 microg/ml and 0.5 microg/ml. The iodide uptakes of FRTL-5 cells were significantly increased at the levels of group 0.33 microg/ml and 0.5 microg/ml in 12 h. Perchlorate sodium made significant decreases of the iodide uptake of FRTL-5 cells in 12h and 24h. CONCLUSION: The mechanism of the thyroid hormone action disrupted by pentachlorophenol may be associated with descreasing thyroglobulin in FRTL-5 cells.