Effect of inhibition of inducible nitric oxide synthase (iNOS) on the murine splenic immune response induced by Aggregatibacter (Actinobacillus) actinomycetemcomitans lipopolysaccharide |
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Authors: | Sosroseno W Musa M Ravichandran M Ibrahim M F Bird P S Seymour G J |
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Institution: | School of Dentistry, AIMST University, Kedah Darul Aman, Malaysia;;Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu, Malaysia;;Department of Microbiology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu, Malaysia;;School of Dentistry, Universiti Sains Malaysia, Kota Bharu, Malaysia;;Oral Biology and Pathology, School of Dentistry, University of Queensland, St Lucia, Queensland, Australia;;Faculty of Dentistry, The University of Otago, Dunedin, New Zealand |
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Abstract: | Animal studies suggest that inducible nitric oxide synthase (iNOS) may be associated with destructive periodontal disease. l- N 6-(1-Iminoethyl)-lysine ( l -NIL) has been shown to inhibit iNOS in a selective manner, and hence the aim of the present study was to test the hypothesis that treatment with l -NIL may induce a T-cell helper 1 (Th1)-like immune response by Aggregatibacter ( Actinobacillus ) actinomycetemcomitans lipopolysaccharide (LPS)-stimulated murine spleen cells in vitro . BALB/c mice were either sham-immunized or immunized with A. actinomycetemcomitans LPS. Spleen cells were stimulated with A. actinomycetemcomitans LPS in the presence or absence of l -NIL. Nitric oxide (NO), iNOS activity, specific IgG subclass antibodies, interferon- γ (IFN- γ ), and interleukin-4 (IL-4) levels and cell proliferation were determined. The results showed that treatment with l -NIL suppressed both NO production and iNOS activity but enhanced specific IgG2a, IFN- γ levels, and increased cell proliferation following stimulation with A. actinomycetemcomitans LPS-stimulated cells. The results of the present study suggest that inhibition of iNOS activity by l -NIL may skew the A. actinomycetemcomitans LPS-stimulated murine splenic immune response towards the Th1-like immune profile in vitro . |
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Keywords: | A actinomycetemcomitans l-NIL LPS murine spleen |
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