| 丹参诱导骨髓基质干细胞向神经元样细胞分化及相关基因的表达 |
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| 引用本文： | 陆长青,王勇,陈登榜,郭琼,冉黎,王凡. 丹参诱导骨髓基质干细胞向神经元样细胞分化及相关基因的表达[J]. 四川解剖学杂志, 2006, 14(1): 1-4 |
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| 作者姓名： | 陆长青 王勇 陈登榜 郭琼 冉黎 王凡 |
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| 作者单位： | 四川大学华西基础与法医学院人体解剖教研室,四川,成都,610041;四川大学华西基础与法医学院生物医学工程教研室,四川,成都,610041 |
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| 摘 要： | 目的分析骨髓基质干细胞向神经元样细胞分化前后相关基因的表达。方法用丹参诱导骨髓基质干细胞向神经元样细胞分化,诱导后90mins,提取诱导前后的骨髓基质干细胞总RNA,RT-PCR检测ngn-1,mash-1的表达及观察其诱导前后的表达情况,免疫组化检测NSE和GFAP的表达情况。结果未经诱导的骨髓基质干细胞ngn-1,mash-1 mRNA为阴性,诱导后有表达。诱导后的细胞NSE和GFAP呈阳性反应。结论骨髓基质干细胞向神经元样的分化与ngn-1,mash-1可能有关。
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| 关 键 词： | 骨髓基质细胞 基因表达 神经元样细胞 丹参 |
The Gene Expression in Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells into Neurocyte-Like Cells Inducing by Danshen |
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| Lu Chang-Qing,Wang Yong,Chen Deng-Bang,Guo Qiong,Ran Li,Wang Fan. The Gene Expression in Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells into Neurocyte-Like Cells Inducing by Danshen[J]. Sichuan Journal of Anatomy, 2006, 14(1): 1-4 |
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| Authors: | Lu Chang-Qing Wang Yong Chen Deng-Bang Guo Qiong Ran Li Wang Fan |
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| Affiliation: | 1 The Departerment of Anatomy; 2 The Department of Biomedicine Engineering, School of Preclinical and Forensic Medicine ,Sichuan University, Chengdu 610041 China |
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| Abstract: | Objective To investigate the gene expression of Mash-1 and Ngn-1 in differentiation of SD rat bone marrow-derived mesenchymal stem cells inducing by danshen.Methods We isolated the nucleated cells from rat bone marrow through gradient centrifugation,then induce MSCs differentiation into neurcyte-like cells by Danshen,detected the expression of specific enolase(NSE) and glial fibrillary acidic protein(GFAP) by immuncytochemistry.RT-PCR was used to detect the mRNA of Mash-1 and Ngn-1.Results The expression of Mash-1 and Ngn-1 do not detected before the inducing by danshen,and can found expression after the inducing.Both NSE and GFAP were immuncytochemistrical positive.Conclusion Mash-1 and Ngn-1 might play an important role when the MSCs differentiation into neurocyte-like cells inducing by danshen.. |
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| Keywords: | Bone marrow-derived mesenchymal stem cells Gene expression Neuron-like cells Danshen |
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