微生物降解对人参自毒作用的缓解效应

连作障碍问题严重制约人参生产及土地资源的合理利用,自毒作用是导致连作障碍的关键因子之一。在前期筛选到人参自毒物质降解菌的基础上,研究采用平板培养法和常规生理生化方法测定人参种子萌发的生物学指标和保护性酶活性,以探究自毒物质降解菌对人参自毒作用的缓解效果。研究发现,除棕榈酸外,自毒物质苯甲酸、邻苯二甲酸二异丁酯、丁二酸二异丁酯和2,2-(4-羟基苯基)丙烷降解液对人参种子生长的毒害作用得到显著缓解,与对照无显著差异。除苯甲酸外,其余自毒物质降解后人参SOD,POD,CAT酶活性均较降解前显著降低。研究结果表明,微生物降解是缓解自毒物质抑制人参种子萌发的有效途径,该发现将有助于人参连作障碍问题的解决。

Mitigative effect of micribial degradation on autotoxicity of Panax ginseng

Continuously cropping obstacle restricts ginseng production and rational use of land resource severely, and autotoxicity is one of the most important factors. In our previous work, ginseng autotoxin degrading bacteria were isolated, in the present research, plate culturing method and traditional physiological and biochemical method were used to analyze biological indices and protective enzyme activities, in order to elucidate the mitigative effect of autotoxin degrading bacteria on autotoxicity of P. ginseng. Results indicated that, except for palmitic acid, autotoxicity of benzonic acid, diisobutyl phthalate, diisobutyl succinate, and 2,2-bis (4-hydroxyphenyl) propane on the growth of ginseng seeds was significantly alleviated after autotoxins degrading bacteria was inoculated, and which have no evident difference with control. Except for benzoic acid, enzyme activity of SOD, POD and CAT in other autotoxin degrading treatments decreased significantly. The present research showed that, microbial degradation could alleviate the autotoxicity of autotoxins on ginseng seeds effectively, and which will be helpful for the resolution of ginseng continuously cropping obstacle problem.