胰岛移植物质量鉴定方法的研究

胰岛移植物的质量直接影响胰岛移植的效果，用经胶原酶消化，Ｆｉｃｏｌｌ纯化的成年大鼠胰岛，比较乙醇和二甲亚砜配制的双硫腙，作Ｂ细胞鉴别染色，丫啶橙－碘丙啶和双醋酸荧光素－溴乙锭双色荧光染色，鉴别活细。

Methods for evaluation of the islet graft quality

The identification of beta cell and living cells of purified islets of adult rats were comparatively studied with ethanol or DMSO prepared dithizone (DTZ) and with acridine orange (AO) propidium iodide (PI) or fluorescent diacetate (FDA) ethidium bromide (EB) respectively. The results indicated that the function and viability of islet cells were not influenced by the staining, the insulin content of the culture medium and the insulin release test of the stained islet were not significantly different from those of the unstained controls ( P > 0.05 ). The cell toxicity of ethanol prepared DTZ was much less than that of DMSO prepared. In addition, the ethanol prepared DTZ was easily preserved. AO PI staining could identify the apoptotic cells because the binding of AO with double chain DNA showed green fluorescence, while the binding of AO with single chain DNA (degenerated DNA) showed red fluorescence. Therefore, DTZ and AO PI combined staining may exactly identify the purity, amount and viability of the islets.