雷公藤甲素对白血病HL-60和Jurkat细胞增殖及凋亡的影响

目的:探讨雷公藤甲素对人急性髓系白血病HL-60细胞、急性T淋巴细胞白血病Jurkat细胞增殖及凋亡的影响。方法:采用MTT法、Annexin V-FITC/PI双染法、PI染色法、透射电镜观察不同浓度雷公藤甲素作用于两种细胞,对其生长增殖、凋亡、细胞周期及形态等方面的影响。结果:雷公藤甲素能显著抑制HL-60、Jurkat细胞的增殖,降低其存活率。给药24 h后,半数细胞抑制剂量(IC50)分别为(42.50±9.38)nmol/L和(60.91±9.77)nmol/L。能以剂量依赖方式诱导两种细胞凋亡,细胞周期分布G1期比例增加,S期比例降低(P<0.05),且伴有典型的细胞凋亡形态学改变。结论:雷公藤甲素能显著抑制HL-60、Jurkat细胞的生长增殖,并诱导细胞凋亡。

Effects of triptolide on the proliferation and apoptosis of human leukemia HL-60 and Jurkat cells

Objective:To determine the effect of triptolide on the proliferation and apoptosis of HL-60 cells in acute myeloid leukemia and Jurkat cells in T-cell acute lymphoblastic leukemia. Methods: Human leukemia cell lines were treated with triptolide at different concentrations. The inhibitory rate of cells proliferation was detected by MTT assay, apoptosis rate was detected by AnnexinV-FITC/PI double staining, cell cycle was observed by PI staining, and cell morphology was observed by transmission electron microscope (TEM). Results: Cell proliferation of HL-60 and Jurkat cells was significantly inhibited and their survival rates were reduced by triptolide in a dose-dependent manner, with the IC50 value for 24 h of (42.50 ± 9.38) nmol/L and (60.91 ± 9.77) nmol/L, respectively. Triptolide induced cells apoptosis in a dose-dependent manner,apparent cell cycle and typical apoptotic morphological changes. Conclusion: Triptolide could significantly inhibit cell proliferation and induce apoptosis in human leukemia cell lines of HL-60 and Jurkat.