D101型大孔树脂纯化山豆根总生物碱的工艺优选

目的:优选D101型大孔树脂纯化山豆根总生物碱的工艺条件。方法:选择苦参碱为指标成分,采用UV测定总生物碱含量,检测波长415 nm。以总生物碱含量为指标,通过单因素试验考察上样量质量浓度、洗脱剂用量及种类、洗脱流速等因素对山豆根总生物碱大孔树脂纯化工艺的影响。结果:最佳工艺条件为上样液用盐酸调p H 3~4,用20%氨水调p H 10,上样液质量浓度0.8 g·m L-1,上样流速2 BV·h-1,上样量为山豆根药材与树脂体积比例1∶2.5,加水2 BV除杂,用70%乙醇4 BV于4 BV·h-1流速洗脱;总生物碱纯度66.83%。结论:D101型大孔树脂对山豆根生物碱类物质具有较好的纯化效果,优选的工艺简单可行,为山豆根的资源利用提供参考

Optimization of Purification Technology of Total Alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 Macroporous Resin

Objective: To optimize purification technology conditions of total alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 macroporous resin. Method: Taking matrine as index component, UV was employed to determine the content of total alkaloids with detection wavelength of 415 nm.With the content of total alkaloids as index, effects of the concentration of sample solution, amount and type of eluent, elution velocity on purification process were investigated by single factor tests. Result: Optimum technology conditions were as follows:adjusted pH of sample solution to 3-4 with with hydrochloric acid, adjusted pH to 10 with 20% ammonia water, the concentration of sample solution 0.8 g·mL^-1, sample flow rate of 2 BV·h^-1, sample volume(equivalent as crude drug)-resin volume ratio of 1: 2.5, washed impurity with 2 BV of water, with 4 BV of 70% ethanol as eluant, elution flow rate of 4 BV·h^-1.Purity of total alkaloids was 66.83%. Conclusion: D101 macroporous resin has good purification effect for total alkaloids from Sophorae Tonkinensis Radix et Rhizoma, optimized process is simple and feasible, this study provides a reference for resource utilization of Sophorae Tonkinensis Radix et Rhizoma.