黔产粗毛淫羊藿与黔岭淫羊藿总黄酮对2型糖尿病小鼠IR相关因子的影响

目的:研究黔产粗毛淫羊藿与黔岭淫羊藿中总黄酮(TFE)对四氧嘧啶(ALX)诱发2型糖尿病模型小鼠胰岛素抵抗(IR)相关因子的影响。方法:雄性小鼠100只,随机取10只为正常组,喂养基础饲料;其余的采用高脂饲料喂养小鼠6周,单次ip 2%ALX(200 mg·kg-1),建立2型糖尿病小鼠模型。将糖尿病模型小鼠随机分为6组,每组10只,分别为模型组,二甲双胍(150 mg·kg-1)组,两种TFE分别按剂量100,50 mg·kg-1分组,ig给药14 d。于末次给药后取血,检测小鼠血清胰岛素(FINS)、C肽水平;检测小鼠空腹血糖(FBG),注射胰岛素(Ins)40 min后血糖(BG);计算胰岛素耐量(ITT),胰岛素抵抗指数(HOMA-IR)和胰腺指数;观察小鼠胰腺形态学改变。结果:与正常组比较,模型组小鼠血糖升高非常显著(P0.01),空腹血清中C肽,FINS有明显增加(P0.05),胰腺组织损伤极为严重(P0.01),胰腺指数降低非常显著(P0.01)。与模型组相比,两种淫羊藿TFE均能够较明显地改善模型小鼠ITT(P0.05),降低模型小鼠血清C肽、FINS含量及HOMA-IR(P0.05),提高模型小鼠的胰腺指数(P0.05),明显地修复模型小鼠的胰腺组织损伤(P0.05)。两种TFE同剂量组相比,以粗毛淫羊藿TFE作用较为显著(P0.05,P0.01)。结论:两种黔产淫羊藿TFE对ALX诱生的2型糖尿病模型小鼠能增加胰岛素敏感性,较明显地改善模型小鼠胰岛素抵抗相关因子,保护受损的胰腺组织。以粗毛淫羊藿TFE作用较为明显,这可能与粗毛淫羊藿TFE中淫羊藿苷(ICA)含量高有较大关系。

Effects of Total Flavonoe of Epimedium of Two Species in Guizhou on IR-related Factors in Type 2 Diabetes

Objective: To study the effects of total flavonoe of Epimedium(TFE)from Epimedium acuminatum and E. Leptorrhizum grown in Guizhou on insulin resistance (IR)-related factors in mice induced by alloxan(ALX). Method: Hundred male mice were divided into normal group and model group. The mice in model group were fed with high-fat diet for 6 weeks, then single intraperitoneal injection of 2% ALX solution (200 mg·kg^-1) was made to establish type 2 diabetes models.The modeling mice were randomly divided into 6 groups with 10 in each group, including metformin group(150 mg·kg^-1), E.acuminatum TFE group(100, 50 mg·kg^-1), E.leptorrhizum TFE group(100, 50 mg·kg^-1), respectively, once a day for 14 days. After 14 days, the animals were fasted for 12 hours, and killed. Then the level of serum insulin (FINS) and C peptide was determined.The blood sugar (FBG) and BG after injection of ins 40 min were detected. Insulin tolerance (ITT), insulin resistance index (HOMA-IR) and pancreatic index were calculated. HE staining was used to observe the morphologic changes of pancreas in mice. Result: Compared with the normal group, blood sugar of the model group increased significantly(P<0.01), C-peptide and FINS significantly increased(P<0.05), pancreatic tissue injury was serious(P<0.01), the pancreas index decreased significantly(P<0.01). Compared with model group, two TFE from E. acuminatum all obviously improved ITT(P<0.05), pancreatic index(P<0.05), reduced C-peptide, FINS and HOMA-IR(P<0.05) and obviously alleviated pancreatic tissue injury(P<0.05) in mice induced by ALX. TFE from E. acuminatum had more obvious effect(P<0.05, P<0.01). Conclusion: Two kinds of TFE from E. acuminatum have certain effect on the type 2 diabetes mice induced by ALX, and can increase insulin sensitivity, improve IR-related factors on the type 2 diabetes mice, and have a good protective effect on the pancreatic tissue injured. Especially, TFE from E.acuminatum has obvious effect. Epimedium's active ingredient on diabetes may has a greater relationship with icariin.