| 同源导入近交系绿色荧光裸小鼠Foxn1nu.B6-CAG-EGFP/SU的建立 |
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| 引用本文： | 沈艳华,王麒龙,代兴亮,陈金,董军,兰青,黄强.同源导入近交系绿色荧光裸小鼠Foxn1nu.B6-CAG-EGFP/SU的建立[J].中国比较医学杂志,2015,25(1):55-58,65. |
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| 作者姓名： | 沈艳华 王麒龙 代兴亮 陈金 董军 兰青 黄强 |
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| 作者单位： | 苏州大学医学部实验动物中心, 江苏 苏州 215123;苏州大学附属第二医院神经外科, 江苏 苏州 215123;苏州大学附属第二医院神经外科, 江苏 苏州 215123;苏州大学附属第二医院神经外科, 江苏 苏州 215123;苏州大学附属第二医院神经外科, 江苏 苏州 215123;苏州大学附属第二医院神经外科, 江苏 苏州 215123;苏州大学附属第二医院神经外科, 江苏 苏州 215123 |
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| 基金项目： | 国家自然科学基金(81071766、81172400、81272799、81472739);国家自然科学基金"胶质瘤干细胞重构微环境诱导宿主细胞癌变的相关机制研究"(81472739). |
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| 摘 要： | 目的建立一个能稳定表达增强型绿色荧光蛋白(enhanced green fluorecence protein,EGFP)的裸小鼠近交新品系,供包括人脑胶质瘤在内的所有人类肿瘤移植实验用。方法将雌性C57BL/6-Tg(CAG-EGFP)转基因小鼠和雄性Foxn1nu裸小鼠,按同源导入方法进行杂交和回交,用荧光手电筒和匹配的眼镜、多功能活体成像仪、荧光显微镜对是否表达EGFP进行鉴别,传至F10后进行遗传学检测。结果所建品系命名为Foxn1nu.B6-CAGEGFP/SU(Soochow University),14个遗传生化位点中13个表型与BALB/c(Foxn1nu)吻合,唯Pep3(肽酶-3)的电泳为b型,而标准的BALB/c近交系为a型;外周血淋巴细胞占全部有核细胞15%,T淋巴细胞仅占0.3%,与亲本的Foxn1nu一致。结论成功地建立了一个既与亲本C57BL/6-Tg N(CAG-EGFP)-样稳定表达EGFP,又与Foxn1nu裸小鼠(BALB/c背景)一样具有T细胞缺乏的荧光裸小鼠新品系-Foxn1nu.B6-CAG-EGFP|SU,Pep3为b型是有别于Foxn1nu裸小鼠的生化遗传标记位点。
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| 关 键 词： | 绿色荧光蛋白 转基因小鼠 裸小鼠 近交系 |
| 修稿时间： | 2014/12/3 0:00:00 |
Establishment of a new congenic inbred mouse strain named Foxn1nu.B6-CAG-EGFP/SU |
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| SHEN Yan-hu,WANG Qi-long,DAI Xing-liang,CHEN Jin-sheng,DONG Jun,LAN Qing and HUANG Qiang.Establishment of a new congenic inbred mouse strain named Foxn1nu.B6-CAG-EGFP/SU[J].Chinese Journal of Comparative Medicine,2015,25(1):55-58,65. |
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| Authors: | SHEN Yan-hu WANG Qi-long DAI Xing-liang CHEN Jin-sheng DONG Jun LAN Qing and HUANG Qiang |
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| Institution: | Laboratory animal center of Soochow University, Jiangsu Suzhou 215123, China;The Second Affiliated Hospital of Soochow University, Jiangsu Suzhou 215123, China;The Second Affiliated Hospital of Soochow University, Jiangsu Suzhou 215123, China;The Second Affiliated Hospital of Soochow University, Jiangsu Suzhou 215123, China;The Second Affiliated Hospital of Soochow University, Jiangsu Suzhou 215123, China;The Second Affiliated Hospital of Soochow University, Jiangsu Suzhou 215123, China;The Second Affiliated Hospital of Soochow University, Jiangsu Suzhou 215123, China |
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| Abstract: | Objective To establish a new congenic inbred mouse strain carrying and expressing EGFP and Foxn1nugene for cancer research including human glioma as well. Methods According to criterion of GB14923-2010, the male Foxn1nu nude mice backcross the female C57BL/6-Tg (CAG-EGFP) transgenic mice for 10 times, Then identify the phenotype using the methods and equipment as below: fluorescent flashlight and matching glasses; multifunction vivo imager; fluorescence microscopy. Results The congenic inbred mouse strain named Foxn1nu. B6-CAG-EGFP/SU (Soochow University). All the 14 biochemical loci are homozygous and same with Balb/c mouse in addition to the Pep3 loci ("b" type instead of "a" type). Peripheral blood lymphocyte count shows the lymphocytes occupy 15% of nucleated cells; T lymphocytes occupy 0.3%, meet the requirement of inbred strain of EGFP nude mice. Conclusions Established a new congenic inbred strain -Foxn1nu.B6-CAG-EGFP/SU which both express EGFP stably, and own immunodeficiency with lack of T lymphocytes. The phenotype "b" of biochemical loci "Pep3" is the unique characteristic that distinguish SU to Foxn1nu. |
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| Keywords: | Green fluorescent protein Transgenic nude mice Nude mouse Inbred strain |
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