| 乌头碱给药大鼠尿液生物标记物的检测 |
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| 引用本文： | 辛杨,张滢,张哲,张伟光,吕君,王丽艳,徐蕾.乌头碱给药大鼠尿液生物标记物的检测[J].中国实验方剂学杂志,2015,21(3):89-91. |
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| 作者姓名： | 辛杨 张滢 张哲 张伟光 吕君 王丽艳 徐蕾 |
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| 作者单位： | 齐齐哈尔大学化学与化学工程学院, 黑龙江 齐齐哈尔 161006;齐齐哈尔大学化学与化学工程学院, 黑龙江 齐齐哈尔 161006;齐齐哈尔大学化学与化学工程学院, 黑龙江 齐齐哈尔 161006;齐齐哈尔大学化学与化学工程学院, 黑龙江 齐齐哈尔 161006;齐齐哈尔大学化学与化学工程学院, 黑龙江 齐齐哈尔 161006;齐齐哈尔大学化学与化学工程学院, 黑龙江 齐齐哈尔 161006;齐齐哈尔大学化学与化学工程学院, 黑龙江 齐齐哈尔 161006 |
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| 基金项目： | 国家自然科学基金项目(81403067);黑龙江省教育厅科技研究项目(12541874);齐齐哈尔市科技局社会发展指导性计划项目(SFZD-2012087) |
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| 摘 要： | 目的:筛选乌头碱给药大鼠尿液的生物标记物,寻找该化合物的相关代谢通路。方法:20只Wister大鼠随机分为正常组与给药组,采用液液萃取法处理生物样品,利用超高效液相色谱-四级杆/飞行时间质谱检测正、负离子模式下空白组大鼠与给药组大鼠尿液中内源性代谢物,通过主成分分析法对2组大鼠生物样品进行无监督模式的评价,筛选对分类起关键作用的内源性代谢物,并寻找可能相关的代谢通路。结果:共筛选出5个对分类起主要作用的内源性代谢物,得到结构表征的有4个,分别为propionylglycine methyl ester,Cys-Ser-Me,5,8-dihydroxy-3,4-dihydrocarbostyril,吡咯-2-羧酸。结论:乌头碱的阵痛作用可能与体内五羟色胺分泌量的降低有关,为乌头碱的作用机制研究提供参考。
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| 关 键 词： | 乌头碱 超高效液相色谱-四级杆/飞行时间质谱 主成分分析 生物标记物 吡咯-2-羧酸 代谢组学 |
| 收稿时间： | 2014/12/8 0:00:00 |
Detection of Rats' Urine Biomarkers of Aconitine Poisoning by UPLC/Q-TOF Mass Spectrometry |
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| XIN Yang,ZHANG Ying,ZHANG Zhe,ZHANG Wei-guang,LYU Jun,WANG Li-yan and XU Lei.Detection of Rats' Urine Biomarkers of Aconitine Poisoning by UPLC/Q-TOF Mass Spectrometry[J].China Journal of Experimental Traditional Medical Formulae,2015,21(3):89-91. |
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| Authors: | XIN Yang ZHANG Ying ZHANG Zhe ZHANG Wei-guang LYU Jun WANG Li-yan and XU Lei |
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| Institution: | College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China |
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| Abstract: | Objective: To screen urine biomarkers of rats after administrated aconitine and find out its related metabolic pathways. Method: Wister rats were divided into two groups which corresponded to control group(CG) and medication administration team (MAT), respectively.In each group, there were five male rats and five female rats.Biological samples were handled by liquid-liquid extraction method.Ultra high performance liquid chromatography (UPLC) coupled with time of flight mass spectrometry (TOF-MS) was applied, positive and negative ion mode was used to monitor endogenous metabolites in urine samples of rats, principal component analysis was applied to distinguish biological samples, biomarkers were selected by partial least square discriminant analysis and found out related metabolic pathways of aconitine. Result: Four potential biomarkers were identified, which were propionylglycine methyl ester, Cys-Ser-Met, pyrrole-2-carboxylic acid and 5, 8-dihydroxy-3, 4-dihydrocarbostyril. Conclusion: This study indicates that analgesia of aconitine may be associated with reduction of serotonin secretion, which can provide research basis for further study on mechanism of aconitine poisoning. |
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| Keywords: | aconitine UPLC-Q/TOF-MS principal components analysis biomarkers pyrrole-2-carboxylic acid metabonomics |
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