Evaluation of the WIDER I system for antimicrobial susceptibility testing of clinical isolates of Haemophilus influenzae and Streptococcus pneumoniae |
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| Authors: | F. Ferná ndez-Cuenca,L. Martí nez-Martí nez,A. Pascual,M. De Cueto,O. Gutié rrez,J. Nieto, E. J. Perea |
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| Affiliation: | Department of Microbiology, University of Seville, Apdo. 914, 41009, Seville and;Francisco Soria Melguizo, SA, Madrid, Spain;Tel: +34 95 5008287 Fax: +34 95 4377413 E-mail: |
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| Abstract: | The aim of this study was to evaluate the WIDER I system for susceptibility testing of Haemophilus influenzae and Streptococcus pneumoniae . MICs of 12 antimicrobials against 42 H. influenzae and 58 S. pneumoniae strains were determined using 1W MIC panels and compared with those obtained by microdilution. Overall essential agreements were >99%. Very major errors were not detected. Major errors occurred with ampicillin (1.7% H. influenzae ). Minor errors were 2.3% (amoxicillin–clavulanate, cefuroxime, chloramphenicol), 7.1% (ampicillin) and 16.7% (clarithromycin) for H. influenzae , and 1.7% (chloramphenicol, erythromycin, meropenem), 3.4% (amoxicillin–clavulanate, cefuroxime, tetracycline) and 8.6% (levofloxacin) for S. pneumoniae . The WIDER I system is a reliable method for susceptibility testing of H. influenzae and S. pneumoniae . |
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| Keywords: | Antimicrobial susceptibility testing automated systems Haemophilus influenzae Streptococcus pneumoniae |
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