| 全血与血液制品中HCV RNA检测及其基因分型的初步研究 |
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| 引用本文： | 李方和,郭林生.全血与血液制品中HCV RNA检测及其基因分型的初步研究[J].中华微生物学和免疫学杂志,1994,14(4):246-248. |
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| 作者姓名： | 李方和 郭林生 |
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| 作者单位： | 同济医科大学附属同济医院临床免疫研究室 |
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| 基金项目： | 国家科委“八、五”攻关计划资助 |
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| 摘 要： | 对一组临床应用的全血（配血用标本）、血液制剂与丙种球蛋白制剂进行检测。血浆制剂抗ＨＣＶ阳性率显著高于全血（３２．６４％与１１．６８％、Ｐ＜０．０１）。三组标本中抗ＨＣＶ阳性者ＨＣＶ ＲＮＡ检出率基本相似。但丙球制剂组ＨＣＶ ＲＮＡ阳性标本在ＰＣＲ中的反应强度显著低于前两者。对部份标本作ＨＣＶ基因亚型检测，三组总体ＨＣＶ亚型表达频率为，原型：２．９４％；Ｋ１：４７．０６％；Ｋ２ａ：３２．３５％；Ｋ２
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| 关 键 词： | 丙型肝炎 全血 血液制品 基因分型 |
A PRELIMINARY STUDY ON DETECTION OF HCV RNA AND HCV RNA SUBTYPES IN WHOLE
BLOOD AND SOME BLOOD PRODUCTS |
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| Li Fang-he,Guo Ling-shen,Yu Zhi-qun,et al.A PRELIMINARY STUDY ON DETECTION OF HCV RNA AND HCV RNA SUBTYPES IN WHOLE
BLOOD AND SOME BLOOD PRODUCTS[J].Chinese Journal of Microbiology and Immunology,1994,14(4):246-248. |
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| Authors: | Li Fang-he Guo Ling-shen Yu Zhi-qun |
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| Abstract: | e have detected anti-HCV,HCV RNA and HCV RNA subtypes of whole blood
,fresh human plasma and r-globulin all of which were from rural blood donors in Wuhan
area.The results showed that the detection rate of an-ti-HCV in pooled plasma was higher than
that of whole blood group (32. 46%and 11.68%,P<0.01).The resultsof HCV RNA detection rates in
all anti-HCV positive samples in the three groups were similar ,but a weaker reac-tion in PCR
assay had been shown in the r-globulin group.For detecting HCV RNA subtypes,the rates of
variousHCV genetic subtypes were2.94%in HCV Pt,47.06%in HCV K1,32.35%in HCV K2a,14.71%
in HCV K2band2.94%in HCV K3. |
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| Keywords: | Hepatitis C Anti-HCV HCV RNA HCV RNA subtype PCR |
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