mRNA expression and RNA editing (2451 C-to-U) of IL-12 receptor beta2 in adult atopic patients |
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Authors: | Kim Eun Jin Lee Won Mok Ha Jung Sook Ryoo Nam Hee Jeon Dong Suk Kim Jae Ryong |
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Affiliation: | Daegu Kyoungbook Blood Center, Korea Red Cross, Daegu, Korea. |
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Abstract: | Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta(2) subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta(2) mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta(2) mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy. |
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Keywords: | interleukin-12 receptor IL-12Rβ2 Gene Expression Profiling Atopy Asthma Dermatitis Atopic RNA Editing |
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