非小细胞肺癌组织中PTEN/p27kip1的表达及其意义

目的检测PTEN、p27kip1、cyclin 3种蛋白在非小细胞肺癌组织的表达及与临床病理特征的关系.方法在60例非小细胞肺癌原发灶(包括37例鳞癌和23例腺癌)中应用免疫组织化学S-P法检测3种蛋白的表达.结果 60例肺癌原发灶中PTEN、p27kip1、cyclin 3种蛋白表达阳性率分别为:48.3%;41.7%;73.3%.PTEN与淋巴结转移显著相关(<0.05)p27kip1、cyclin 与淋巴结转移无关(>0.05)这3种蛋白表达均与肿瘤细胞分化程度显著相关(<0.05)与组织学类型无关(>0.05)60例非小细胞肺癌组织中,PTEN的表达与p27kip1呈显著正相关(P<0.01),而与cyclin 无明显相关(>0.05)p27kip1与cyclin 的表达之间呈显著负相关(P<0.01).结论 PTEN明显地抑制了非小细胞肺癌的浸润和转移,p27kip1表达缺失与肺癌细胞的分化有关,PTEN/p27kip1衰老诱导途径在非小细胞肺癌的恶性进展中起着很重要的作用.

PTEN/p27kip1 expression and their significance in non-small cell lung cancer

Objective The relationships between the expressions of PTEN, p27 kip1 , cyclin E proteins and the clinicopathological characteristic of NSCLC were investigated. Methods Immunohistochemical methods were applied to detect the expression of PTEN, p27 kip1 , cyclin E in 60 surgical specimens form NSCLC patients (37 squamous cell carcinomas and 23 adenocarcinomas). Results In 60 NSCLC, the positive rates of PTEN, p27 kip1 and cyclin E proteins were 48.3%, 41.7% and 73.3%, respectively.The positive expression of PTEN protein was highly associated with lymph node metastasis( P <0.05), but not the p27 kip1 and cyclin E expressions( P >0.05). Positive rates of these three proteins were closely associated with the cellular differentiation ( P <0.05), but not with the pathological types( P >0.05). PTEN expression was positively associated with p27 kip1 expression( P <0.01),but not with cyclin E expression ( P >0.05). An inverse correlation was present between cyclin E and p27 kip1 expressions( P <0.01). Conclusion PTEN obviously inhibits invasion and metastasis of NSCLC; loss expression of p27 kip1 is correlated with lung cancer cell differentiation, PTEN/p27 kip1 senescence inducing pathway may play an important role in NSCLC malignant progression.