3种常用抗癌药物对宫颈癌Hela细胞和肿瘤干细胞抑制作用研究

目的:探讨3种常用抗肿瘤药物对宫颈癌Hela细胞和Hela肿瘤干细胞的抑制率。方法:采用无血清悬浮培养法从宫颈癌Hela细胞中分离出Hela肿瘤干细胞,鉴定后研究丝裂霉素、顺铂和紫杉醇等3种抗癌药物对Hela细胞和Hela肿瘤干细胞的抑制率并进行比较。结果:不同质量浓度的3种抗肿瘤药物对肿瘤细胞和肿瘤干细胞均有抑制作用,且呈剂量依赖性(r=0.944,P<0.05)。在最高终浓度40 mg/L时,丝裂霉素对肿瘤细胞和肿瘤干细胞的抑制率分别为(88.75±2.59)%和(38.95±5.37)%;顺铂的抑制率分别为(92.28±1.57)%和(52.68±3.55)%;紫杉醇的抑制率分别为(75.22±0.99)%和(36.71±2.42)%。结论:3种药物对肿瘤干细胞的抑制率均明显低于肿瘤细胞的抑制率(P<0.05);比较发现对于肿瘤细胞和肿瘤干细胞,顺铂抑制率均最高,其次是丝裂霉素,紫杉醇的抑制率最低(P<0.05),说明宫颈癌肿瘤干细胞对目前常用的3种治疗药物有不同程度的耐受。

Clinical studies on the effect of three common antitumor drugs on established Hela cervical cancer cell lines and tumor stem cells

Objective： To investigate the effect of three common antitumor drugs on established Hela cervical cancer cell lines and tumor stem cells from the Hela cell lines. Methods： The tumor stem cells were separated and cultured by serum-free medium. Then explore the effect of mitomycin,cisplatin and paclitaxel on Hela cell lines and tumor stem cells separated from Hela cell lines.Results： Different concentration of the three antitumor drugs inhibited the proliferation of tumor cells and tumor stem cells in a dose-dependent manner（ r = 0. 944,P 0. 05）. The inhibitory rates of mitomycin,cisplatin and paclitaxel on Hela cell lines were（ 88. 75 ± 2. 59） %,（ 92. 28 ±1. 57） % and（ 75. 22 ± 0. 99） % respectively; The inhibitory rates on the tumor stem cells were（ 38. 95 ±5. 37） %,（ 52. 68 ± 3. 55） % and（ 36. 71 ± 2. 42） % respectively. Conclusion： The inhibitory rates of the three kinds of drugs on tumor cells were significantly higher than that of tumor stem cells（ P 0. 05）. According to the results,we find that cisplatin has the highest inhibitory rate,while the paclitaxel is the lowest on both Hela cell lines and tumor stem cells（P0.05）.