Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions |
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Authors: | Barbara W. Adam,Dana L. Chafin,Victor R. De Jesú s |
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Affiliation: | Centers for Disease Control and Prevention (CDC), 4770 Buford Highway NE, Atlanta, GA 30341, USA |
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Abstract: | ObjectiveWe aimed to measure separately the contributions of heat and humidity to changes in levels of hemoglobins A and S in dried-blood-spot (DBS) samples.Design and methodsWe stored paired sets of DBSs at 37 °C for predetermined intervals in low-humidity and high-humidity environments. Hemoglobin A and S levels of all samples in each complete set were measured in a single high performance liquid chromatography run.ResultsDuring the one-month storage intervals, both hemoglobin species lost about 35% of initial levels in low-humidity storage and almost all of initial levels in high-humidity storage.ConclusionsMinimizing both humidity and temperature in the transportation and storage environments of DBS samples is essential to maintaining the integrity of the hemoglobin tetramer molecules. |
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Keywords: | Newborn screening Sickle cell disease Hemoglobin S Hemoglobin A Dried blood spots Hemoglobin stability Storage temperature Storage humidity |
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