人胎脑室下区发育过程中神经干细胞的变化

目的观察人胎脑室下区不同发育时期神经干细胞(NSCs)的变化,为调控自身NSCs治疗神经系统退行性疾病提供实验依据。方法收集胎龄24~28周的水囊引产胎儿30例,采用原位杂交及光镜技术对其脑室下区NSCs的分布、形态以及生长方式进行检测。结果不同胎龄人胎脑室下区均可见Nestin mRNA表达阳性NSCs,NSCs呈星形,有3~6个突起,多个突起形成网丛,细胞散在分布在网丛中,胞核呈圆形,1~4个核仁,细胞染色质疏松。多数NSCs单个存在,可见对称分裂的NestinmRNA表达阳性NSCs和3个NSCs形成的集落样生长方式。不同胎龄Nestin mRNA表达阳性NSCs的分布部位、细胞形态无明显区别,但Nestin mRNA表达阳性NSCs生长方式上存在一定差异。结论不同胎龄人胎脑室下区均存在NSCs,NSCs生长方式随胎龄不同而变化,调控在体NSCs有望成为治疗儿童神经退行性疾病的另一种有效方法。

Changes of Neural Stem Cells in Subventricular in Developing Human Fetal Brain at Different Ages

Objective To observe the changes of neural stem cells(NSCs) in subventricular in developing human fetal brain at(diffe)-rent ages.Methods Thirty cases of embryoes at gestational age 24-28 weeks induced by labor with water bag were collected to determin distribution,shapes and growth modes in subventricular with hybridization in situ under light microscope.Results NSCs expressing Nestin mRNA existed in subventricular from human fetal brain at different ages,NSCs existed in subventricular of different fetal age included astro-NSCs,each had enations from 3 to 6,and all projections crowded together into a reticular plexiform,in which NSCs districuted.Nucli were round in shape,each had nucleoli from 1 to 4.NSCs had rarefaction chromatin,most NSCs existed in a single growth mode,symmetral cleavage growth mode and clony with 3 NSCs would be seen.There were no differences between positive Nestin mRNA NSCs in distribution and cell shapes,but had differences in growth mode.Conclusion NSCs exist in subventricular from different gestational ages and their growth mode are changing with difference of gestational age.