OX40L在C57BL/6与BALB/c小鼠组织的差异性表达及分析

背景:有研究发现C57BL/6小鼠对动脉粥样硬化易感,而BALB/c小鼠却对动脉粥样硬化不易感.OX40L的表达情况与动脉粥样硬化的狭窄程度和心肌梗死的严重度相关,其在两种品系小鼠中是否存在表达差异?目的:分析0X40L在BALB/c和C57BU6小鼠心脏、大脑、肾脏、骨骼肌和脾脏组织的表达差异.方法:取C57BL/6和BALB/c小鼠心脏、大脑、肾脏、骨骼肌和脾脏组织,以Trizol提取总RNA, RIPA Buffer提取组织总蛋白.采用RT-PCR和Westem Blot方法检测两种品系小鼠心脏、脑、肾脏、脾脏和骨骼肌的OX40LmRNA和蛋白的表达.OX40L在两种品系小鼠不同器官间的表达差异.结果与结论:RT-PCR结果显示,C57BL/6小鼠心脏OX40LmRNA表达显著高于BALB/c小鼠(P＜0.05),脾脏OX40LmRNA表达明显低于BALB/c小鼠(P＜0.05),两种品系小鼠大脑、肾脏及骨骼肌OX40LmRNA表达差异无显著性意义;Western Blot结果显示,两种品系小鼠OX40L的蛋白表达均在心脏最高;C57BL/6小鼠心、脑及肾OX40L蛋白表达均显著高于BALB/c小鼠(P＜0.05),两种品系小鼠骨骼肌和脾脏OX40L蛋白表达差异无显著性意义.两个品系小鼠OX40L mRNA的表达水平与蛋白表达水平不完全一致.C57BL/6小鼠心脏中OX40L mRNA转录水平较BALB/c高,但在脾脏中表达量较后者低;C57BL/6小鼠心脏、大脑和肾脏OX40L蛋白水平均较BALB/c小鼠高;两种品系小鼠之间的表达差异提示OX40L可能与C57BL/6小鼠易感动脉粥样硬化有关.

Differential expression of OX40L in C57BL/6 and BALB/c mouse tissues

BACKGROUND: Previous studies found that C57BL/6 mouse was susceptible to atherosclerosis, while BALB/c mouse was resistant to atherosclerosis. The stenosis of the culprit vessel and the severity of myocardial infarction were correlated to the levels of OX40L expression. Whether OX40L has differential expression between C57BL/6 and BALB/c mouse was not identified. OBJECTIVE: To observe the differential expression of OX40L mRNA and protein in C57BL/6 and BALB/c mouse. METHODS: Total RNA and protein were extra...