HPLC法测定不同产地夏枯草中熊果酸的含量

目的:建立以高效液相色谱法测定夏枯草中熊果酸含量的方法,并检测不同产地夏枯草中熊果酸的含量。方法:色谱柱为ZorbaxSBC18(150mm×4.6mm,5μm),流动相为乙腈-磷酸盐缓冲液(pH7.6)=45∶55,检测波长为210nm,流速为1.0mL·min-1。结果:熊果酸进样量在0.1115～1.7840μg范围内与峰面积积分值呈良好的线性关系(r=0.9992);平均回收率为99.93%,RSD=0.77%(n=6)。不同产地夏枯草中熊果酸的含量在0.08%～0.18%之间。结论:不同产地夏枯草中熊果酸含量差异很大。本方法简便、准确、重现性好,可用于夏枯草的质量控制。

Determination of Ursolic Acid in Spica Prunellae from Different Habitat by HPLC

OBJECTIVE:To establish an HPLC method for determination of the content of Ursolic acid in Spica Prunellae,and to determine the content of Ursolic acid in Spica Prunellae from different habitat.METHODS:Ursolic acid was separated on Zorbax SB C18(150 mm×4.6 mm,5 μm)with mobile phase consisted of acetonitrile-phosphate buffer(pH 7.6)= 45∶55 at a flow rate of 1.0 mL·min-1.The detective wavelength was set at 210 nm.RESULTS:The linear range of Ursolic acid was 0.111 5～1.784 0 μg(r=0.999 2).The average recovery rate of Ursolic acid was 99.93%(RSD=0.77%,n=6).The content of Ursolic acid in Spica Prunellae from different habitat ranged from 0.08% to 0.18%.CONCLUSION:The content of Ursolic acid in Spica Prunellae from different habitat varied greatly.This determination method is simple,accurate and reproducible,and suitable for the quality control of Spica Prunellae.